本项目拟采用水热／溶剂热法，通过改变基质和掺杂离子，控制上转换纳米材料的生长粒径、晶型，改变掺杂离子的比例，控制反应时间等方法，来制备系列具有长波发射特性的、高发光效率兼具磁性的上转换纳米材料，包括核壳型，掺杂过渡金属离子，以及原位生长金（银）纳米颗粒等复合型纳米材料。通过配体交换或表面吸附等方法对其表面进行生物功能化修饰，使其具有良好的水溶性和生物相容性。利用廉价高效的近红外半导体激光器作为激发光源，结合奥林巴斯荧光倒置显微镜（IX83）, 自组装简便、价廉、高效的基于上转换纳米材料长波发射的成像装置，实现红区-近红外区生物成像等。以多功能长波发射磁性上转换稀土掺杂纳米材料作为探针，建立基于红区-近红外区检测的免疫分析模型等，结合磁性分离、富集，实现抗原、抗体、疾病（例如癌症）标志物等物质的检测以及长波区细胞成像等，以期为发光传感、生物成像等领域提供新材料、新技术、新方法。

In the project, magnetic rare earth doped upconversion nanomaterials with long wavelength emission and high luminescent efficiency will be synthesized through hydrothermal method by doping different rare-earth ions, controlling the crystalline structure and the reaction conditions. The surface of these nanomaterials will be modified by ligand exchange approach. With cheap and efficient semiconductor laser (980nm) used as the light source, a test and imaging platform based on the inverted fluorescence microscope (IX83) will be constructed. With these long wavelength emission and magnetic high luminescent efficiency rare earth doped upconversion nanomaterials as the probe, and the combination of magnetic separation and enrichment technology, several models including immunoassay will be developed for the detection of antigens, antibodies, and tumor markers with high sensitivity and selectivity. The purpose of this plan is to provide new materials, new technology and new methods for luminescence sensing and bio-imaging in the long wavelength region.