本项目拟采用水热法或水热溶剂热法，制备具有良好发光性质和生物相容性的稀土发光材料，包括CePO4:Tb3+、NaYF4: Yb/Er等等。通过控制反应时间，配体的比例，来制备系列具有不同粒径、不同结构的稀土发光材料。通过原位聚合的方法或有机转相的方法对其表面进行功能化改性，使之适宜用作生物标记材料。选择食品中有害微生物如大肠杆菌、金黄色葡萄菌、沙门菌等常见的、具有代表性菌类为研究对象。采用所制备的功能化的稀土发光纳米材料为标记物，通过抗体或核酸适配体进行标记。同时，结合免疫层析技术，制备薄层层析试纸；组装具有紫外光、近红外光（激发装置）以及光电转换装置（检测装置）的小型化扫描、数据处理平台，实现食品中有害微生物的现场、准确、灵敏、快速检测。为食品、保健品中有害微生物的检测提供新材料、新技术、新方法。

In the project, rare earth(RE) luminescent nanomaterials (such as CePO4:Tb3+、NaYF4: Yb/Er) with excellent photostability and biocompartibility, will be synthesized through hydrothermal method and solvothermal method. RE luminescent nanomaterials with different sizes and different structure will be prepared by controlling the reaction time or the concentration of ligand. In order to apply these nanomaterials as biolabeling materials, the surface of these nanomaterials will be modified by the method including in-situ polymerization or transferring from organic solvent to aqueous solution to keep them water-soluble and biocompatible. Several typical harmful microbes in food (such as E. coli, Staphyloccocus aureus Rosenbach, Salmonella) will be selected as subject. The as-prepared RE luminescent nanomaterials will be biolabeled on antibody or aptamer. The immunochromatographic strip will be prepared combined with the technology of immunochromatograghy. A test platform with ultraviolet lamp, near-infrared semiconductor laser and photoelectric converter will be constructed, to develop new method for on site, accurate, sensitive and rapid detection of harmful microbe in food. The aim of this plan is to provide new materials, new technology and new methods for detection of harmful microbe in food and health care products.