糖尿病肾病因其较高的致死致残率给糖尿病患者和社会带来严重危害，临床治疗效果不佳，因此DN的防治非常重要。临床上缩泉益肾方治疗DN有效，但机制尚不明确。外泌体miR-2861的靶基因FOXO1是DN发生过程中重要信号途经INS/PI3K/FOXO1中的一个关键靶点。前期研究发现：益智仁对DN小鼠肾脏中，miRNA-378等2个上调，miRNA-129等5个下调，这些miRNA涉及到与DN相关的多条通路。本研究通过构建抑制miR-2861质粒及转染FOXO1慢病毒载体模型动物及高糖的肾小管上皮细胞，使用缩泉益肾方后，用real-timePCR观察miR-2861及INS、PI3K、FOXO1等mRNA的表达；Western blot、免疫细胞组化、ELISA检测INS/PI3K/FOXO1信号途径关键分子INS、PI3K、FOXO1等蛋白的表达水平，研究缩泉益肾方干预肾阳虚证糖尿病肾病的机制。

Diabetic nephropathy causes serious harm to diabetic patients and society due to its high lethality rate, and it’s clinical treatment effect was not significant.So DN’s preventive treatment is very important.In clinical treatment,suo-quan-yi-shen formula treat DN have effect,But the mechanism is unclear.FOXO1 gene is an important target in the INS/PI3K/FOXO1 signaling pathway,which is belong to exosome miR-2861 target gene.Previous studies have found that The efficacy of fructus alpiniae oxyphyllae for DN mice model’s kidney,will up-regulated two exosomes such as miRNA-378 and down-regulated five exosomes such as miRNA-129.And these miRNA involved multiple pathways related to DN.In this study, we construct the inhibition of mir-2861 plasmid and ransfected FOXO1 lentivirus virus vector model animal and high sugar renal tubular epithelial cells,after use suo-quan-yi-shen formula,we use real-timePCR to observe miR-2861 and INS、PI3K、FOXO1 etc mRNA’s expression;Western blot、Immunohistochem ical staining、ELISA detection INS/PI3K/FOXO1 signal pathway key molecules INS、PI3K、FOXO1 etc.Protein expression level,to research suo-quan-yi-shen formula intervene with kidney Yang deficiency type (syndrome types of TCM) and DN’s mechanism.