糖尿病肾病的早期防治非常重要，临床上以益智仁乌药为主的方剂在肾阳虚型DN早期的多尿期有广泛应用，但机制尚不明确。肾小球系膜细胞增殖是肾阳虚型DN的早期病理改变之一，PTEN/PI3K 在糖尿病肾病的发生发展中是一个关键的信号传导通路。miR-21 的靶基因PTEN 基因是 PI3K信号途径中的一个重要节点。本研究通过构建miR-21质粒，转染到高糖的肾小球系膜细胞及模型动物体内，给益智仁乌药后，以PTEN/PI3K信号通路为切入点，用real-timePCR观察miR-21的表达；Western blot、免疫细胞组化检测PTEN/PI3K信号途径关键分子PTEN、p-Akt和 PI3K p85α的蛋白质表达水平，研究药对益智仁乌药（温补肾阳法）干预肾阳虚型（证）糖尿病肾病（病）的机制，为药对益智仁乌药临床治疗DN提供理论依据，同时丰富以miR-21为切入点的中医药治疗DN的机制研究。

Early prevention and treatment measures are very inportant for diabetic nephropathy (DN). Formulars mainly composed of Yizhi Ren and Wuyao are widely used to treat kindey yang deficiency DN though the mechanism remains unknown.In the occurrence and progression of early kindney yang difiency DN, mesangial cell proliferation is one of the main pathological changes, PTEN/PI3K is the key signaling pathway, while miR-21 target genes PTEN gene is a critical node of the PI3K signaling pathway.This study aims to explore the mechanism of Yizhi Ren and Wuyao treating early kindney yang difiency DN. MiR-21 plasmid were constructed and then transfected into high-sugar mesangial cells and animal model. After administration of Yizhi Ren and Wuyao, miR-21 expression level was determined by real-timePCR according to the observation of PTEN/PI3K signaling pathway; expression levels of proteins PTEN, p-Akt and PI3K p85α which are major molecules of PTEN/PI3K signaling pathway were detected by Western blot and immune cells methods. By this way, we discussed how Tonify kidney yang therapeutic principle works on kidney yang deficiency DN, and provides theoretic evidence for this therapy; it also promotes further related study.