钙调蛋白（CaM）受钙离子信号调控，参与植物生长发育和对多种非生物逆境信号的响应。项目组前期研究发现钙调蛋白GmCaM与高温高湿胁迫下春大豆种子活力的形成相关，本项目拟以GmCaM及其互作蛋白胚胎发育晚期丰富蛋白LEA4和甘油醛-3-磷酸脱氢酶GAPDH为对象，在种子活力形成关键时期：通过过表达转化大豆和基因沉默，明确GmCaM参与高温高湿胁迫下春大豆种子活力形成的作用和功能，以及GmCaM与其互作蛋白受钙信号调控的方式；对高温高湿胁迫条件下GmCaM与蛋白LEA4和GAPDH的互作关系进行深入验证，并揭示其互作方式；综合分析，揭示GmCaM参与田间高温高湿胁迫下春大豆种子活力形成的机制。其结果将从一个侧面为阐明田间高温高湿胁迫下春大豆种子活力形成的机制提供帮助；拓宽对钙信号响应逆境胁迫的调控途径认识，为种用以及商用春大豆优质生产提供调控思路，推动我国南方春大豆生产和加工利用的发展。

Calmodulin (CaM), regulated by Ca2+ signals, is involved in growth and development and in response to various abiotic stresses in plant. GmCaM has been found previously to be related to the formation of seed vigor of spring soybean under high temperature and humidity (HTH) stress in the field in our Lab. In the present project, using GmCaM and its interaction proteins-late embryogenesis abundant protein Lea4 and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as objects of study and in the crucial period of seed vigor formation, firstly, we will make clear how GmCam functions in the formation of seed vigor and how GmCaM and its interaction proteins are regulated by Ca2+ signals under HTH stress through gene silencing and overexpression transformation of soybean. Secondly, we will further validate the interaction relationship between GmCaM and Lea4 as well as GAPDH under HTH stress and elucidate the interaction modes. Thirdly, based on all the above results, we will reveal the mechanism of calmodulin GmCaM participating in seed vigor formation of spring soybean under HTH stress. All the results of this project will be helpful from one side for elucidating the formation mechanism of seed vigor of spring soybean under HTH stress and enrich our understanding of the regulation approaches of Ca2+ signal in response to adversity (HTH stress), which will provide regulation thoughts for the high quality production of seed and commercial soybean.