Quantitative proteomic, physiological and biochemical analysis of cotyledon, embryo, leaf and pod reveals the effects of high temperature and humidity stress on seed vigor formation in soybean
Quantitative proteomic, physiological and biochemical analysis of cotyledon, embryo, leaf and pod reveals the effects of high temperature and humidity stress on seed vigor formation in soybean
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子叶、胚、叶和豆荚的定量蛋白质组学、生理生化分析揭示高温高湿胁迫对大豆种子活力形成的影响
DOI：
10.1186/s12870-020-02335-1
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发表时间：
2020-03
影响因子：
5.3
通讯作者：
Ma H
中科院分区：
文献类型：
--
作者：
Wei JP;Liu XL;Li LZ;Zhao HH;Liu SS;Yu XW;Shen YZ;Zhou YL;Zhu YJ;Shu YJ;Ma H
研究背景大豆种子发育过程中易受田间高温高湿胁迫，导致种子活力下降。实际上，在大豆种子生长发育过程中，田间的HTH也会胁迫整个植株，尤其是叶片和荚果，进而通过营养供应和保护作用影响种子的生长发育和活力形成。摘要从蛋白质、超微结构、生理生化等方面，研究了HTH胁迫对小麦种子活力形成的综合影响。在黄瓜子叶、胚和叶片中分别鉴定出247、179和517个差异丰富蛋白（DAP）。而在湘豆3号的子叶、胚和叶片中分别检测到235、366和479个DAP。宁镇1号。此外，在HTH胁迫下，两个品种的子叶、胚和叶片中分别鉴定出120、144和438个DAP。此外，在HTH胁迫下，两个品种的子叶、胚和叶片中分别鉴定出120、144和438个DAP。已鉴定的大部分DAP参与了植物的主要代谢途径和细胞过程，包括信号转导、三羧酸循环、脂肪酸代谢、光合作用、蛋白质加工、折叠和组装、蛋白质合成或降解、植物-病原体相互作用、淀粉和蔗糖代谢以及氧化胁迫反应。HTH胁迫对湘豆3号4个器官的代谢途径、细胞超微结构和生理生化的负面影响均小于宁珍1号，导致湘豆3号种子活力较高。结论高活力种子的形成主要是通过提高子叶蛋白质的合成和营养物质的储存，提高胚的稳定性和生活力，HTH胁迫下叶片的光合能力和养分供应能力增强，对荚果的保护作用增强。这些结果提供了HTH胁迫下大豆叶片、荚果和种子（子叶和胚）的综合性状，其中一些可作为大豆高活力育种的选择指标。
BackgroundSoybean developing seed is susceptible to high temperature and humidity (HTH) stress in the field, resulting in vigor reduction. Actually, the HTH in the field during soybean seed growth and development would also stress the whole plant, especially on leaf and pod, which in turn affect seed growth and development as well as vigor formation through nutrient supply and protection.ResultsIn the present study, using a pair of pre-harvest seed deterioration-sensitive and -resistant cultivars Ningzhen No. 1 and Xiangdou No. 3, the comprehensive effects of HTH stress on seed vigor formation during physiological maturity were investigated by analyzing cotyledon, embryo, leaf, and pod at the levels of protein, ultrastructure, and physiology and biochemistry. There were 247, 179, and 517 differentially abundant proteins (DAPs) identified in cotyledon, embryo, and leaf of cv. Xiangdou No. 3 under HTH stress, while 235, 366, and 479 DAPs were identified in cotyledon, embryo, and leaf of cv. Ningzhen No. 1. Moreover, 120, 144, and 438 DAPs between the two cultivars were identified in cotyledon, embryo, and leaf under HTH stress, respectively. Moreover, 120, 144, and 438 DAPs between the two cultivars were identified in cotyledon, embryo, and leaf under HTH stress, respectively. Most of the DAPs identified were found to be involved in major metabolic pathways and cellular processes, including signal transduction, tricarboxylic acid cycle, fatty acid metabolism, photosynthesis, protein processing, folding and assembly, protein biosynthesis or degradation, plant-pathogen interaction, starch and sucrose metabolism, and oxidative stress response. The HTH stress had less negative effects on metabolic pathways, cell ultrastructure, and physiology and biochemistry in the four organs of Xiangdou No. 3 than in those of Ningzhen No. 1, leading to produce higher vigor seeds in the former.ConclusionHigh seed vigor formation is enhanced by increasing protein biosynthesis and nutrient storage in cotyledon, stronger stability and viability in embryo, more powerful photosynthetic capacity and nutrient supply in leaf, and stronger protection in pod under HTH stress. These results provide comprehensive characteristics of leaf, pod and seed (cotyledon and embryo) under HTH stress, and some of them can be used as selection index in high seed vigor breeding program in soybean.
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影响因子：
3.4
作者：
Catusse, Julie;Meinhard, Juliane;Job, Dominique
通讯作者：
Job, Dominique
影响因子：
5.6
作者：
Yin X;He D;Gupta R;Yang P
通讯作者：
Yang P
影响因子：
3.3
作者：
Lintong Yang;Y. Qi;Yi-Bin Lu;Peng Guo;W. Sang;H. Feng;Hong-Xing Zhang;Lisong Chen
通讯作者：
Lintong Yang;Y. Qi;Yi-Bin Lu;Peng Guo;W. Sang;H. Feng;Hong-Xing Zhang;Lisong Chen
影响因子：
4.3
作者：
Chu, Pu;Chen, Huhui;Huang, Shangzhi
通讯作者：
Huang, Shangzhi
影响因子：
2.8
作者：
Ken’ichiro Matsumoto;Juri Saito;Toshinori Yokoo;C. Hori;A. Nagata;Yuki Kudoh;T. Ooi;S. Taguchi
通讯作者：
Ken’ichiro Matsumoto;Juri Saito;Toshinori Yokoo;C. Hori;A. Nagata;Yuki Kudoh;T. Ooi;S. Taguchi