研发可用于替换受损/堵塞血管的小口径人工血管具有重要意义。生物电对许多组织的功能维持和损伤修复起重要的调控作用。在构建骨、心脏等支架时引入生物电因素均能有效促进组织再生。但人工血管的构建是否需要考虑生物电因素，以及电刺激在血管再生与功能稳态维持中的作用尚无研究。因此，本项目拟对小口径动脉血管的压电系数及其释放的电信号特征进行分析，为血管材料引入生物电因素提供参数依据。然后，以压电聚合物为原料，通过静电纺丝、熔融纺丝与湿法纺丝技术的单独或联合使用，制备可仿生天然动脉电信号的压电小口径血管材料。在动态培养下，研究压电血管材料释放电刺激对血管细胞和巨噬细胞行为的调控作用及其作用机制。通过动物体内移植，研究压电血管材料释放电刺激对血管组织再生与功能稳态维持的影响。通过体、内外实验的相互验证系统阐明电刺激调控血管再生的信号传导机制。该项目的实施将为血管损伤修复提供新型的小口径人工血管。

There is clear and unmet clinical need to develop functionally superior small-diameter artificial vascular grafts for blood vessel tissue regeneration. Bio-electricity plays an important role in maintaining normal tissue physiological functionality and has been shown to improve the repair of injured tissues. Various applications of bio-electrical factors in bone, heart, and other tissue engineering scaffolds, have proven effective in promoting tissue regeneration. However, the incorporation of bio-electrical factors in the construction of vascular grafts and the considerations of the roles of electrical stimulation on vascular regeneration in this context, have yet to be studied. Therefore, in this study, we aim to measure the piezoelectric coefficient of native artery vessels and analyze the characteristics of generated electrical signals, thus providing parameters to match for the incorporation of bio-electric factors into vascular grafts. Next, we will use pziezoelectric polymers to prepare small-diameter vascular grafts capable of releasing electrical signals comparable to the natural artery tissue. Grafts will be fabricated and optimized through the combined use of electrostatic spinning, melt-spinning and wet-spinning. The regulating effect and action mechanism of electrical stimulation released by piezoelectric vascular grafts on the behavior of vascular cells and macrophages will be studied by using dynamic culture devices. Finally, the animal models for vascular implantation model will be used to evaluate the influence of electrical stimulation released by piezoelectric vascular grafts on vascular patency and tissue regeneration. The regulatorying effects and its signal transduction mechanisms resulting from of electrical stimulation relating to the in regulation of vascular regeneration will can be systematically clarified through the in vivo and in vitro experiments. The implementation of this project will provide a new concept for the construction of small-diameter artificial vascular graft and help promote their development toward clinical studies.