理解和控制纳米材料与生物体系相互作用的探索将促进纳米材料生物医学应用的快速发展。针对相关的蛋白质冠研究中缺乏原位、实时分析方法的难题，本项目将在课题组前期工作的基础上，定制不同厚度、曲率半径、表面性质以及具有衍射光学和局域表面等离子体共振（LSPR）特性的有序多孔纳米结构基底，选择血红蛋白、转铁蛋白和乙肝表面抗原等以及能与其不同抗原决定簇特异性结合的多种抗体作为典型的蛋白质分子作用体系，研究三种蛋白质在基底表面形成蛋白质冠过程中的结构、动力学以及生物学特性等，发展识别蛋白质冠界面上抗原决定簇信息的表征技术，利用衍射、LSPR峰位移定量检测分子结合，获取与分子独特振动能级相对应的拉曼、红外光谱指纹图谱检测和鉴别分子信息，建立基于有序多孔纳米结构基底结合其自身优异光学特性的蛋白质分子相互作用分析新方法，为真正实现纳米材料的生物医学应用提供理论基础。

The search for understanding and controlling the interactions of nanomaterials with living organisms will lead to the rapid development of their biomedical applications. With respect to the absence of in situ and real-time analytical methods in the study of protein coronas, based on our previous work, our project will prepare ordered porous nanostructure substrates with different thickness, radius of curvature, surface properties, optical diffraction and localized surface plasmon resonance (LSPR) properties. Hemoglobin, transferrin, hepatitis B surface antigen, and their antibodies which can specifically bind to the different epitopes will be chosen to construct the typical interaction systems of protein molecules. Structures, kinetics and biological properties during the formation of protein coronas on the surfaces of the substrates will be investigated to develop the characterization technique for the identification of the epitope information on the protein coronas. Diffraction and LSPR peak-shifts will be used to detect quantitatively molecular binding. Raman and IR fingerprints will be obtained corresponding to the specific vibration levels of molecules and used to detect and identify the molecular information. Based on the combination of ordered porous nanostructure substrates with their own excellent optical properties, a new method will be established for the study of the interactions between protein molecules, provide theoretical basis for applying nanomaterials to the biomedical applications.