丰富的天然毛色是羊驼最重要的独特的生物学特性，阐明羊驼毛色形成及基因控制机理，并通过分子生物学手段人工调控动物毛色，使之适合于人类对动物天然毛色的需求具有重大理论和实践意义。MC1R基因是已证明的调控羊驼毛色的最主要基因之一，最新文献及本课题组前期研究发现，G蛋白信号通路中的Gαs基因可能在MC1R基因调控羊驼毛色蛋白表达中起着关键作用。本项目以来自中华羊驼养殖基地的羊驼为研究对象，用分子生物学等技术手段获得Gαs基因，干扰G蛋白相关通路的基因，并获取毛囊干细胞中G蛋白通路对MC1R调控相关毛色基因和毛色相关蛋白的表达，探索在毛囊干细胞中G蛋白信号通路中Gαs基因参与MC1R控制的毛色形成机制，揭示该细胞通路与羊驼毛色形成的关系。从而为阐明动物毛色形成的分子机理、通过分子生物学技术人工调控或改造动物毛色提供科学依据。

Rich natural hair color are the important and unique biological characteristic of alpaca. The clarification of the the regulation mechanism of alpaca hair color formation has important theoretical and practical significance for improving animal hair color. It has been proved that the MC1R gene is one of the most important genes regulating alpaca hair color, the newest references and our previous research has found the Gαs gene （one of the main factors in G-protein way）might play a key role in MC1R regulating hair color protein expression of alpaca. The alpaca from Zhonghua Alpaca Breeding Farm will be used in the project. The project will be on the basis of National Nature Science Fund titled "Influence of MC1R gene on hair color protein expressed by alpaca hair follicle stem cells"(30972223). In this study, Gαs gene was cloned and used to interfernce correlative genes in G-protein way in alpaca. In order to clarify the mechanism of hair color formantion regulated by G protein way via Gαs, and to proclaim the relationship between G-protein pathway and hair color formantion in alpaca, the influences of G-protein way on the expression hair color gene and protein regulated by MC1R was investigated in alpaca hair follicle stem cells was studied. Thus, our results will provide a sceintific foundation for further understanding molecular mechanism of animal hair color formaton and for manipulation animal hair color by molecular biological technology.