水稻稻瘟病抗病机制是植物抗病研究的热点，但广谱抗病及其分子机理报道很少。利用EMS诱变我们获得了一份稻瘟病广谱抗病水稻材料h1006，与其野生型水稻相比，该材料重要农艺性状和产量性状没有明显变化。前期的研究中我们发现编码RNA结合蛋白的基因Tpr1突变后水稻h1006表现出对稻瘟病的广谱抗病性，并且证实了TPR1能够结合RNA。本项目拟利用植物病理、植物生理、分子遗传及分子生化等技术手段研究RNA绑定蛋白TPR1如何通过与其关键互作蛋白TAPs相互作用、相互修饰，来影响参与免疫反应的关键RNA的修饰（如剪切，降解或翻译等），进而影响水稻免疫反应，从而系统阐述TPR1调控稻瘟病广谱抗性的分子机制。由于先前在拟南芥、水稻以及其他植物中尚未有类似的研究和报道，该研究将为广泛利用广谱抗病位点tpr1提高水稻稻瘟病抗性提供新的重大理论基础，还将为其他植物广谱抗病分子机制的研究提供重要借鉴。

Through recent decades of efforts, a lot of progresses have been achieved in light of rice resistance against blast caused by Magnaporthe oryzae (M. oryzae), the most devastating disease for rice. However, broad-spectrum resistance to blast and its underlying mechanism remain largely unknown. We obtained a novel rice blast disease resistant mutant, h1006, through EMS-mediated mutagenesis. The h1006 mutant confers broad-spectrum resistance against M. oryzae without affecting agricultural traits or yield. By using a strategy combining map-based cloning and whole genome sequencing, we identified the tpr1 gene, which encodes an RNA binding protein, and found that disruption of the Tpr1 gene in the h1006 mutant causes broad-spectrum resistance. We propose three aims: (1) isolate one or two TPR1-associated proteins (TAPs) and one or two TPR1-associated RNAs (TARs); (2) elucidate the molecular regulation by TPR1 and TAPs on modification of key immunity-related TARs, including cleavage, degradation, and mistranslation; (3) characterize the biological roles of TAPs and TARs on tpr1-mediated blast resistance. Through these studies, we will uncover the molecular mechanism regulating broad-spectrum resistance mediated by the TPR1 RNA binding protein. Because there are no similar reports in plants previously, our work will broaden basic knowledge in understanding and applying broad-spectrum resistance to rice blast and possibly other plant diseases. Our work will also provide an important model of studying broad-spectrum resistance in other crops.