基于生物浸出的典型嗜酸热古菌适应含硫底物和活化硫的分子机制研究

本申请立题研究基于生物浸出的典型嗜酸热古菌适应含硫底物和活化硫的分子机制。主要针对黄铜矿及其生物浸出过程中形成的中间含硫产物，通过综合分析典型嗜酸热古菌适应氧化含硫底物过程中细胞表面/胞外多聚物的诱导表达差异、含硫底物表面的硫的分子形态变化，以及微生物数量和群落结构（混合菌浸出时）演变及微生物硫氧化关键酶基因表达差异及变化，表征嗜酸热古菌适应含硫底物的过程机制及其对体系硫氧化效应的关系；筛选嗜酸热古菌表面硫活化关键蛋白质，克隆表达代表性硫活化关键蛋白质，研究其硫活化作用，推衍其可能的作用结构模型。通过这些研究揭示典型嗜酸热古菌适应含硫能源底物和活化元素硫的分子机制。为进一步阐明嗜酸热古菌硫生物氧化机理奠定理论基础，同时为调控生物浸出过程中嗜酸热古菌硫氧化活性提供理论依据。

This item is to research the molecular mechanism of bioleaching relevant adaptation to sulfur-containing substrates and activation of sulfur by typical thermoacidophilic archea (TA). By mainly focusing on chalcopyrite and the sulfur-containing intermediates formed during bioleaching of chalcopyrite, it investigates the induced differential expression of the surface/extracellular polymer substances, and the surface sulfur speciation of sulfur-containing substrates，and as well the succesions of microbial population and community structures (when bioleaching with mixed cultures) and differential expressions of genes for the key sulfur oxidation enzyme(s) during TA's adaptating and oxidizing sulfur substrates, and on which to characterize the adaptation process and therein the effects of it on the spcific sulfur oxidation efficiency of the bioleaching systems. It also screens the key sulfur activation relevant surface proteins (KSARSP) of TA, clones and expresses the representative KSARSP, and evaluate the sulfur activation action and derives the functional structural model of KSARSP. It is expected by these researches to demonstrate the molecular adaption mechanism of the TA to the sulfur-containing energy substrates and the molecular mechanism of sulfur activation of TA, and lay the theory bases for further evaluating the sulfur oxidation mechanism of TA, and regulating the sulfur oxidation activity during bioleaching with TA.