家蚕Ng基因的精细定位及粘液腺分泌相关基因的筛选与鉴定

昆虫在产卵时由其粘液腺分泌的胶着物质附着在卵的表面，将卵固定在寄主植物或产卵材料，可以对昆虫卵期生存及幼虫孵化起到保护作用。为研究家蚕粘液腺分泌功能及其机理，本研究以正常家蚕和产不胶着卵的粘液腺突变体（Ng）为材料，对Ng基因进行精细定位，测定正常家蚕及Ng突变体的粘液腺转录组、蛋白质组，通过对正常家蚕粘液腺及其Ng突变体粘液腺的转录组与蛋白质组比较关联分析，筛选、鉴定一些差异表达的基因；克隆相关差异基因、原核表达并制备抗体，采用免疫组织化学和荧光定量PCR，明确家蚕粘液腺相关差异基因的表达模式，分析这些基因与家蚕粘液腺发育及其分泌功能关系，阐明家蚕分泌胶着物质的分子机理，为从分子水平上揭示家蚕粘液腺的发育提供参考；解释家蚕粘液腺中特异表达的基因对昆虫的生殖调控机制，探讨以昆虫蚕粘液腺及其特异表达的基因作为害虫防治的新靶标，为对害虫的防治研究提供新思路、新方法。

The insect eggs are fixed on the host plants or other materials that can protect eggs through secreting adhesive proteins to the egg surface. In this study, the normal silkworm and Ng mutant will be used as material. we will observe the morphological and ultrastructure changes of silkworm colleterial gland during development period, The transcriptome and proteome of normal and Ng mutant will be sequenced and identified. The differentially expressed genes are screened and identified by comparison of correlation analysis of transcriptome and proteome. Then cloning the related genes, then follow prokaryotic expression and antibody preparation. The expression pattern of the related genes will be definited by immunohistochemistry and fluorescence quantitative PCR. the relationship between the related genes and secretion function of silkworm colleterial gland will be analysed to clarify the molecular mechanism of secreting adhesive proteins.The result will provide references for revealing the silkworm colleterial gland development at the molecular level, which can explain the reproductive regulation mechanism of the related gene of insects colleterial. On these basis we will investigate a new target by using the colleterial gland and its related genes which can provide new ideas and methods for pest control.