转移是影响结直肠癌患者生存率的重要因素。研究表明，肌醇与肌醇六磷酸联用可以显著提升肌醇六磷酸的抗肿瘤效果。本课题组前期通过脾膜注射法建立结直肠癌肝转移小鼠模型，发现二者联用可以有效地抑制结直肠癌肝转移的发生。但肌醇六磷酸和肌醇合用在结直肠癌转移的全过程中所起的作用及其机制尚不明确。本项目拟通过建立原位移植结直肠癌转移小鼠模型，全面系统观察二者联用对结直肠癌从原发到转移全过程的抑制作用，结合体外多种人源细胞培养实验，采用蛋白组学、基因沉默、PCR及Western Blot等方法，研究二者联用通过调节免疫、肿瘤微环境及上皮细胞间质化抗肿瘤转移的作用机制及关键靶点；通过流行病学调查，采用病例-对照研究，探讨膳食因素，特别是肌醇六磷酸与肌醇摄入量与结直肠癌转移风险之间的关系。该项目将会为确定肌醇六磷酸和肌醇的抗肿瘤转移作用提供全面的证据，为预防和治疗结直肠癌转移提供新的思路。

Metastasis is an important factor affecting the outcome of colorectal cancer patients. The combination of inositol (Ins) and Inositol Hexaphosphate (IP6) can significantly enhance the anti-tumor effect of IP6. Our preliminary study proved that the combination of IP6 and Ins effectively inhibited the occurrence of colorectal cancer liver metastasis using a liver metastasis model of colorectal cancer in BALB/c mice which were injected with tumor cells in the spleen. However, the role of IP6 and Ins in the whole process of colorectal cancer metastasis and its mechanism remain unclear.The present project aims to establish a mouse model of orthotopic implantation tumor of colorectal cancer to comprehensively observe the inhibitory effect of the combination of IP6 and Ins on the whole metastastic process of colorectal cancer, combined with a variety of in vitro human cell experiments. Experimental methods such as proteomics, gene silencing, PCR, Western Blot will be used to explore the anti- metastatic mechanism and the key targets of the combination of IP6 and Ins by adjusting the immune, tumor microenvironment and epithelial mesenchymal transition. Through epidemiological investigation, a case-control study will be utilized to explore the relationship between colorectal cancer metastasis risk and dietary factors, especially IP6 and Ins intake . The project will provide more comprehensive evidence for the antitumor metastasis of IP6 and Ins and insights into the prevention and treatment of colorectal cancer metastasis.