昆虫翅发育调控机制研究具有重要的科学意义和良好的害虫生防应用前景。去泛素化酶UBPY在翅发育过程中起重要的调控作用，但其作用机理仍然未被充分理解。已有报道表明UBPY可以影响Wingless及Hedgehog信号通路，而我们发现UBPY能够调控多个与翅发育相关的重要信号分子。我们还发现UBPY通过调节转运必需内体分选复合物ESCRT-0亚基Hrs的稳定性来调控内体运输途径。可见UBPY在翅发育过程中处于比较上游的调控地位，其功能的重要性和复杂性需要深入研究。模式昆虫黑腹果蝇是长期用于遗传学和发育生物学研究，是探索UBPY生物学功能的理想材料。本项目将在果蝇中集中研究UBPY调控昆虫翅发育的关键科学问题，包括：UBPY对不同发育信号通路调控的分子机制；UBPY调控ESCRT-0复合体稳态的具体机理；UBPY活性的调控方式。通过上述研究，希望能揭示UBPY对昆虫翅发育的综合调控作用机理。

Understanding the regulatory network during insect wing development is scientifically important and has great potential in pest control. The deubiquitinating enzyme UBPY is essential for insect wing development, but the underlying mechanism is not fully understood. It has been reported that UBPY can modulate Wingless and Hedgehog signaling pathways. We have shown that UBPY is able to regulate multiple signaling molecules which are required for wing development. We also found that UBPY stabilizes Hrs protein, an essential subunit of the ESCRT-0 (endosomal sorting complex required for transport-0) complex. The importance and complexity of UBPY’s role during wing development awaits further investigation. Drosophila melanogaster is widely used as a model insect for genetics and developmental studies. We propose to investigate the molecular mechanism of UBPY during wing development in Drosophila. The specific scientific problems we aim to pursue are: how UBPY regulates multiple developmental signaling pathways; how UBPY regulates the homeostasis of ESCRT-O complex; and how the activity of UBPY is regulated. Our proposed studies will help to reveal the integrated role of UBPY in regulation of insect wing development.