逆转录酶抑制剂类药物是治疗人获得性免疫缺陷病毒（HIV）感染的主要途径，但药物长期应用会使HIV 产生耐药性。本申请项目将研究该类药物对同是逆转录病毒的禽白血病病毒（ALV）在体外和体内的抑制作用，构建ALV 在相关药物及其组合的选择压作用下的连续传代系列，并对不同传代毒株的不同基因特别是pol 基因扩增测序、比较分析病毒准种的演变动态，以探索药物选择压下ALV 耐药毒株产生的可能性及相关的基因变异。本研究不仅可作为研究抗病毒药物对HIV 等逆转录病毒及其演变的分子机制的动物模型，也可为我国许多原种鸡场实施ALV 净化过程中辅助药物控制手段提供实验依据。

The use of combinations of reverse transcriptase inhibitor drugs have proven remarkably effective in controlling the progression of human immunodeficiency virus (HIV) disease, but these benefits can be compromised by the development of drug resistance. This project attempt to study the inhibitory effect of these drugs to another retrovirus Avian leukosis virus (ALV) in vitro and in vivo and construct a passage serial of ALV under the selective pressures of these drugs, aiming to find the mutation of different gene especially for pol gene and the evolution of it’s quasispecies. This research can be used as a good model to study the evolution molecular mechanism of some retroviruses such as HIV, also can supply some basic data for the possible effect for these drugs using in the eradication program of breed chickens in China.