癸醛抑制果蔬采后青霉属致病菌的分子机制研究

癸醛是一种安全、高效、广谱并且环保的抑菌物质，对果蔬采后青霉属致病菌Penicillium expansum（梨果类采后主要病害）和Penicillium digitatum（柑橘类采后主要病害）具有明显的抑制作用。本文首先通过生理、生化及相关分子指标的检测，确定了癸醛对Penicillium expansum和Penicillium digitatum生长发育及致病力的影响；然后通过Small RNA-sequencing、mRNA-sequencing、Isobaric tag for relative and absolute quantitation结合生物信息学分析筛选出癸醛在病原菌转录组及蛋白质组水平的作用靶标；接着利用pCAMBIA 1300-HPH双元载体以及农杆菌介导的转化验证部分关键作用靶标基因的生物学功能，最终阐明癸醛抑制果蔬采后青霉属致病菌的分子机制。

Decanal is a kind of safe, efficient, broad-spectrum and environment friendly inhibitive substance. It has obvious inhibitory effect on Penicillium expansum, the main postharvest pathogen of pome fruit and Penicillium digitatum, the main postharvest pathogen of citrus fruit. Both of them are major postharvest pathogen and quite harmful to fruit and vegetable preservation. In this study, the effects of decanal on growth and pathogenicity of P. expansum and P. digitatum were determined via assessments of physiological, biochemical and related molecular indexes firstly. Acting sites of decanal at transcriptome and proteome level in pathogen were then determined by techniques of Small RNA-sequencing, mRNA-sequencing, Isobaric tag for relative and absolute quantitation combining bioinformatics analysis. After that, The binary vectors of pCAMBIA 1300-HPH and agrobacterium mediated transformation were constructed to validate the biological function of some key target genes. Finally, the molecular mechanism of decanal against Penicillia was clarified.