Identification of differentially expressed genes involved in spore germination of Penicillium expansum by comparative transcriptome and proteome approaches.
Identification of differentially expressed genes involved in spore germination of Penicillium expansum by comparative transcriptome and proteome approaches.
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通过比较转录组和蛋白质组方法鉴定与扩展青霉孢子萌发相关的差异表达基因
DOI：
10.1002/mbo3.562
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发表时间：
2018-06
期刊：
影响因子：
3.4
通讯作者：
Lai T
中科院分区：
文献类型：
--
作者：
Zhou T;Wang X;Luo J;Ye B;Zhou Y;Zhou L;Lai T
本研究从自然侵染的苹果果实中分离到一种常见的破坏性植物病原菌和棒曲霉产生菌--扩展青霉，并对其进行了形态观察和rDNA内转录间隔区分析。随后，用RNA-seq(RNA测序)和iTRAQ(相对和绝对定量等压标签)方法对大白杨孢子萌发过程中转录组和蛋白质组变化的整体情况进行了评估。共鉴定出3,026个差异表达基因(DEG)、77个差异表达的预测转录因子和489个差异表达蛋白(DEP)。下一步包括通过RNA-seq和iTRAQ数据集之间的相关性分析筛选出130个重叠的候选者。部分基因在mRNA和蛋白质水平上表现出不同的表达趋势，其中大部分参与代谢和遗传信息处理。这些结果不仅突出了一组在破译膨胀杨种子萌发的分子过程中起重要作用的基因和蛋白质，而且为开发有效的控制方法和适当的环境条件奠定了基础。
In this study, Penicillium expansum, a common destructive phytopathogen and patulin producer was isolated from naturally infected apple fruits and identified by morphological observation and rDNA‐internal transcribed spacer analysis. Subsequently, a global view of the transcriptome and proteome alteration of P. expansum spores during germination was evaluated by RNA‐seq (RNA sequencing) and iTRAQ (isobaric tags for relative and absolute quantitation) approaches. A total of 3,026 differentially expressed genes (DEGs), 77 differentially expressed predicted transcription factors and 489 differentially expressed proteins (DEPs) were identified. The next step involved screening out 130 overlapped candidates through correlation analysis between the RNA‐seq and iTRAQ datasets. Part of them showed a different expression trend in the mRNA and protein levels, and most of them were involved in metabolism and genetic information processing. These results not only highlighted a set of genes and proteins that were important in deciphering the molecular processes of P. expansum germination but also laid the foundation to develop effective control methods and adequate environmental conditions.
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