为了大幅提高桑黄纤孔菌液体发酵效率，探索其液体发酵产物的合成规律，本研究拟从真菌激发子的筛选入手，利用植物病原真菌和木腐真菌（包括白腐真菌和褐腐真菌）作为真菌激发子的备选菌株来源进行筛选；通过分析真菌激发子组成、多糖分子量分布、单糖组成、糖苷键类型及糖环结构等，明确不同种类及成分来源的真菌激发子对桑黄纤孔菌黄酮生物合成的影响；对于筛选出的高效真菌激发子，通过响应面分析的方法，对其添加时间、激发时间和添加量等参数进行优化，获得优化的激发条件。该研究将为桑黄纤孔菌黄酮增产研究提供一条新思路，并为这一宝贵药用真菌资源的开发利用提供良好的理论基础和技术支撑。

Fungal elicitor was widely used in medicinal plants, especially in cell suspension culture, to improve the secondary metabolite production of flavonoids, terpenoids, alkaloids and saponins etc., and induce new secondary metabolites production. The secondary metabolite production of medicinal mushrooms has high pharmacological activities, but the application of fungal elicitor in medicinal mushrooms was rarely reported previously. Recently, the study about fungal elicitor to medicinal mushroom secondary metabolites was limited. So the fungal elicitor screening and structure-activity relationship remains to be investigated. In order to greatly improve the efficiency of liquid fermentation of Inonotus sanghuang and explore the synthesis rule of flavonoids, besides the common plant pathogenic fungi, the wood rot fungi (including white rot fungi and brown rot fungi) was added as the alternative strains for fungal elicitor screening. To detect the effects of fungi elicitor types and composition on the biosynthetic of Inonotus sanghuang flavonoids and possible structure-activity relationship, the polysaccharide composition, molecular weight distribution, monosaccharide composition, glycosidic bond type and sugar ring structure of fungal elicitor will be analysed. Furthermore, response surface method will be used to optimum excitation conditions, including the adding time, firing time and add quantity etc. This study will provide a new idea to Inonotus sanghuang flavonoids stimulation and a promising theoretical basis and technical support for the development of this kind of valuable medicinal mushroom.