长白山地区胡桃楸根中萘醌类成分及其诱导肺癌细胞凋亡机制的研究

肺癌是世界范围内死亡率最高的恶性肿瘤之一，从低毒性的天然药物中研制抗肺癌药物成为新药开发的热点。在前期工作中，利用荧光素酶报告基因及MTT法筛选出了长白山地区胡桃楸根的乙醇提取物不仅具有明显的抗肿瘤作用，还能促进TNF-α诱导A549细胞凋亡。因此，已经对活性部位采用各种色谱手段分离得到16个萘醌类化合物（2个新化合物）。本项目在此基础上拟运用先进的分子生物学技术，通过活性追踪的方法寻找活性成分，进而从蛋白质和RNA等不同层面，探究其活性化合物对NF-κB信号通路上几个重要因子与细胞凋亡关系，揭示肺癌疾病中细胞凋亡的作用机理。本课题的研究对象和所挖掘的信息，不仅为研制抗肺癌新药提供新的先导化合物，亦将对长白山地区优势资源的综合利用具有十分重要的意义。

Currently, lung cancer is one of the malignant tumors with the highest mortality around the world.Development of natural anti-lung cancer drugs from the less toxicnatural sources is becoming hot light. Our previous research indicated, the ethanolic extract of the roots of Juglans mandshurica Maxim around Changbai Mountain not onlyshowed significantly anti-tumor effect, but also promoted TNF-α-induced apoptosis of A549 cells. Based on the preliminary work, we have been isolated 16 quinones (2 new compounds) from the active fractions. Following up previous research, the purposal of this study is further bioguided isolation to yield activity compounds by phytochemistry and molecular biology. Subsequently, the mechanisim of bioactive compounds will be performed targeting apoptosis and important cytokines in NF-κB signaling pathway with the different layers of proteins and RNA molecular biology techniques.The result of this research can establish the foundation for exploitation of anti-lung cancer agents from Chinese folk medicine around Changbai Mountain area.