典型抗生素抗性基因在近海网箱养殖海藻生态修复中的分布转移机制研究

抗生素抗性基因(Antibiotic Resistance Genes, ARGs) 作为一类新型环境污染物在海水养殖环境中的持续性残留比抗生素残留本身对生态环境的危害更大，而海水网箱养殖大型海藻修复长期作用下抗生素抗性基因类型、水平及转移的影响及机制的信息相对缺乏。为此，项目结合野外采样与实验室研究，综合传统微生物学手段和现代分子生物学技术定量PCR、宏基因组分析等，拟在大型海藻修复网箱养殖沉积物富集污染系统中，分析不同大型海藻及其不同栽培密度下系统微生物群落改变特性，结合富集的四环素类抗生素迁移转化规律，重点分析大型海藻与富集抗生素联合诱导压力对微生物群落多样性及其已有tet基因类型、分布选择及转移的的选择效应，以为大型海藻支撑可持续海水网箱养殖的技术发展提供一定保障。

Antibiotic resistance genes (ARGs) are emerging micropollutants with environment persistence, having more harmful effects on ecosystem than those of antibiotics. In view of little information of ARGs fates and its' horizontal transfer characteristics during seaweed bioremediation in marine cage culture, this program is designed to study the mechanism of accumulated microbial antibiotic resistance genes (ARGs) variation during seaweeds bioremediation of polluted marine cage culture environment. Based on field sample testing and lab-scale experiments, combined conventional microbial technology and modern molecular biotechnology involving RTQ-PCR and Metagenomic analysis, characteristics of microbial community dynamic is determined in seaweeds bioremediation of marine culture sediment. In consideration of accumulated tetracyclines removal performances during bioremediation, the effect mechanisms of seaweeds and its’ co-existence with tetracyclines on existing tet genes behavior, distribution and transfer are further explored. The results of this program will be helpful for developing sustainable marine cage culture.