近年来，动物分子系统学研究越来越集中在属下、近缘种间以及种下的小尺度范围内。在这些进化尺度下，传统的线粒体与核基因分析已经无法提供足够的信息。序列捕获与RAD-seq是近年来流行的两种高通量研究技术，但序列捕获的探针合成非常昂贵，且一般仅适用于高阶元的系统学研究，RAD-seq方法又只能在近缘种或种下水平应用，目前属内远缘物种间的系统关系研究目前缺少一种通用的高通量测序分析技术。针对这一问题，本项目拟通过限制性酶切片段长度筛选与生物素杂交探针的扩增制备，开发一种新的简化基因组捕获技术（SIG capture），使其成为适用于属内远缘物种间分子系统学研究的一种通用且经济的高通量研究方法。此外，本项目还拟利用SIG capture方法解析中国东南部湍蛙属物种间的系统关系，对其物种有效性、分类地位、种组划分等进行讨论，进一步证明SIG capture方法在解析属内远缘物种系统关系中的效能。

In recent years, animal molecular phylogenetic studies at small scale of evolution, such as within genus, among close-related species and intra-species, has become increasingly common. In such small evolutionary scales, traditional mitochondrial and nuclear genes normally can not provide enough information to resolve phylogenetic relationships of interested. Sequence capture and RAD-seq are two kinds of popular high-throughput sequencing methods for phylogenetic studies of recent years. However, the probes of sequence capture are very expensive, and the sequence capture method is generally suitable for higher-level phylogenetic studies; on the other hand, the RAD-seq method is only applicable for shallow-scale questions, such as among close-related species and intra-species. Currently, there is a lack of a universal high-throughput sequencing method to study phylogenetic relationships of distantly related species within a genus. To solve this problem, this project plans to use size-selected restriction fragments to generate home-made biotin probes, to develop a new simplified genome capture technology (we call it SIG capture). This method will become a new universal and inexpensive high-throughput method to resolve phylogenetic relationships of distantly related species within a genus. In addition, our project will use the SIG capture method to study the phylogenetic relationships of Amolops species of southeastern China, which will provide new insights for species validity, taxonomy, species group division of Amolops. We hope the SIG capture method will become a powerful tool for future phylogenetic studies of small scale of evolution.