由弧菌感染引起的疾病是影响鲍鱼存活率的主要因素。与其他无脊椎动物类似，鲍鱼利用先天性免疫反应来抵御病原微生物的侵染，但目前关于鲍鱼的抗菌免疫机理却研究较少。本项目组前期的转录组测序结果表明，基质金属蛋白酶1（MMP-1）极有可能参与皱纹盘鲍的抗副溶血弧菌免疫反应，但其在鲍鱼免疫应答反应中的作用及其具体作用机制仍不清楚。本项目拟在已有研究的基础上，首先分析皱纹盘鲍MMP-1表达与副溶血弧菌感染的关系；进而利用RNA干扰技术敲低皱纹盘鲍MMP-1的表达水平，研究MMP-1对弧菌感染鲍鱼的影响以及皱纹盘鲍先天性免疫反应相关生理指标的变化情况；最后通过MMP-1互作蛋白的筛选以及下游信号通路的检测来揭示MMP-1参与皱纹盘鲍抗菌免疫的具体作用机制。本项目的研究有助于了解皱纹盘鲍抗弧菌感染的分子机理，也可为鲍鱼的病菌防治策略提供新的思路与途径。

As a common pathogen of abalone, Vibrio infection caused mass deaths of abalone and great economic losses. Among them, Vibrio parahaemolyticus is the most harmful pathogen in abalone aquaculture. It is well known that the disease resistance of abalone, as an invertebrate, is entirely dependent on the innate immune systems, including cellular and humoral responses. To date, little is known about the functional genes and proteins in anti-bacterial immune response of abalone. Our previous study showed that the expression level of matrix metalloproteinase 1 (MMP-1) gene was significantly upregulated in abalone hemocytes when challenged with Vibrio parahaemolyticus, indicating that MMP-1 might be engaged in the anti-Vibrio immune response of abalone. In this project, the relationship between MMP-1 expression and Vibrio parahaemolyticus infection of abalone will be examined. By using RNA interference technology, the effects of MMP-1 on Vibrio parahaemolyticus infection and immune related-physiological activities of abalone will be further explored. Besides, the action mechanism of MMP-1 involved in the immune response of abalone will be investigated through the screening of its interacting proteins and the exploration of downstream signaling pathways. Therefore, our study will be helpful to elucidate the mechanism of anti-pathogen immune response of abalone and serves as an important pathogen-control reference for abalone aquaculture.