捻转血矛线虫是牛羊重要的消化道寄生线虫，其抗药性是全球范围的严重问题，因此疫苗开发迫在眉睫。该虫的天然糖蛋白复合物H11能够诱导宿主产生高水平免疫保护，但是重组蛋白保护效果却大大降低从而阻碍了疫苗的开发。相关研究提示糖基化修饰对抗原免疫原性十分重要，然而，迄今为止对于H11蛋白复合物的糖基化修饰特征及其对免疫保护影响几乎是一无所知。为此，本项目拟全面分析H11糖基化修饰特点并重点探究其与糖基化相关的免疫保护作用。拟选择最新的质谱技术确定其蛋白质组成、聚糖结构、糖基化位点以及糖基化肽段序列，并通过系统生物信息学分析、H11糖基化相关的免疫保护性功能验证以及CHO细胞系重组糖蛋白的免疫保护实验来深入探究糖基化修饰对H11重要糖蛋白的免疫保护功能的影响。本课题的完成将不仅阐明糖基化修饰对于H11蛋白质复合物免疫保护性的影响作用，并可为进一步疫苗的开发奠定坚实基础，具有重大学术意义及重要实践意义。

Haemonchus contortus is an important gastro-intestinal nematode of ruminants. Its drug resistant problem is a global serious problem, therefore vaccine development is imminent. The native H11 protein complex can induce protective immunity in host, however, the efficacy of protective immunity of recombinant forms dramatically decreased, which hampered the development of a commercial vaccine. It is well known know that glycosylation of proteins is very important to immunogenicity of antigens, however, almost nothing is known about glycosylation of H11 and its effect on protective immunity. This project is aimed at comprehensively analyzing the characteristics of glycosylation and its relationship with protective immunity. The most advanced mass spectrometry techniques will be employed to determine the components of protein subtypes, the structure of glycan, sites of glycosylation and glycosylated peptide sequences. In addition, systematic bioinformatics analyses, animal experiments to validate the protective immunity of glycosylated proteins and recombinant proteins produced by CHO cell lines to be used in animal protective experiments will be carried out to elucidate the effect of glycosylation of H11 on the protective immunity. The complement of this project will not only elucidate the effect of glycosylation of the protective immunity of H11 protein complex, and also provide important foundation for further vaccine development, therefore having both academic significance and practical implications.