多数中药的药效成分都属于植物次生代谢物质。植物次生代谢共定位、共表达基因簇的存在，为研究药用植物关键酶新基因、解析代谢途径提供了一条新的道路。丹参是我国重要的根类药材，其药效成分丹参酮合成下游途径至今尚未明确。.项目组利用新组装的丹参基因组数据，发现在1号染色体的长、短臂各存在一个与二萜类物质合成相关的TPS/CYP450和TPS/2OGD基因簇，推测其在丹参酮合成途径起到重要作用。本研究将对这两个基因簇利用转录组、荧光定量技术分析其时空表达特征，结合共表达模式筛选与药效成分合成相关的关键基因；利用异源表达、转基因等方法验证这些候选基因在丹参酮合成途径中的功能；利用BS-Seq、ChIP-Seq、Hi-C结合RNA-Seq数据，从表观遗传学角度解析调控基因簇表达调控的分子机理。本研究不仅将为解析丹参重要药效成分合成途径奠定基础，同时也可为其它药用植物研究提供借鉴。

Most major medicinal effective ingredients of Chinese herb plant are belong to secondary metabolites. The co-located and co-expressed gene clusters involved in secondary metabolic pathway provide novel method to discover key enzyme gene and further elucidate whole metabolic pathway in Chinese herb plant study. Salvia miltiorrhiza Bunge is one of the most important root medicinal herbs with root in China, however its tanshinone synthesis downstream pathway has not been unclear yet. The discovery of plant secondary metabolic gene clusters (MGCs) provided a new direction for mining key enzyme genes and analyzing its synthesis pathway. MGCs related to diterpene synthesis was found in a high-quality S. miltiorrhiza genome obtained by our group. In this study, using transcriptome data and fluorescence quantitative analysis, we will analyze tissue expression characteristics and screen candidate genes and MGCs related to tanshinone synthesis with the co-expression pattern. Heterologous expression and transgene will be used to verify the function of these candidate genes in the synthetic pathway. The regulation mechanism of chromatin spatial conformation, genome-wide methylation and histone modification on MGC expression by combining BS-Seq, ChIP-Seq and Hi-C analysis with RNA-Seq data. This study is expected to mine 1-2 key enzyme genes in tanshinone synthesis pathway which will lay an important foundation on tanshinone synthesis downstream pathway, and provide reference for related study of other medicinal plants.