卵子成熟过程中染色体精确配对分离和胞质不对称分裂是胚胎正常发育的先决条件。纺锤体是卵子减数分裂过程中的动态结构。纺锤体正确组装和迁移保证了染色体正常分离和胞质不对称分裂。我们的前期研究结果发现，胞质分裂调控蛋白1（Protein Regulating Cytokinesis 1, PRC1）在纺锤体形态异常的卵子中严重缺失。进一步结果表明，PRC1通过参与卵子纺锤体组装和迁移过程影响染色体分离和胞质分裂。抑制或过表达PRC1会导致染色体错排、极体排放异常。但是关于PRC1调控卵子染色体运动和胞质不对称分裂的详细分子机理尚不清楚。本项目将利用免疫印迹、活细胞示踪、超高分辨成像、功能干扰与过表达、卵子特异性基因敲除小鼠构建等技术，研究PRC1调控卵子染色体分离和胞质不对称分裂的机理；利用Co-IP、蛋白质谱等技术，揭示PRC1互作分子及位点，为临床筛查异倍性卵子提供依据。

During oocyte meiosis maturation, precise control of chromosome pairing and segregation as well as asymmetric division is the prerequisite for embryo development. Spindle is a temporary dynamic structure during oocyte meiosis. Spindle’s proper organization and migration ensures the accurate chromosome separation and asymmetric cytokinesis. Our preliminary results showed that in oocytes with abnormal spindles, protein regulating cytokinesis 1 (PRC1) was severely deficient. We further found that PRC1 participated in chromosome separation and cytokinesis through controlling spindle organization and migration in oocyte. Inhibition or overexpression of PRC1 leads to defect of chromosome alignment and abnormal of polar body extrusion. However, detailed mechanism of PRC1 regulating chromosome dynamic and asymmetric cytokinesis during oocyte meiosis has not been investigated. This project intends to investigate the potential mechanisms of PRC1 in regulating chromosome dynamic and asymmetric cytokinesis by employing approaches of western blot, living cell image, ultra-high resolution microscopy image, functional impair and overexpression, as well as oocyte specific gene knockout mouse. Co-IP and proteomic approach will be employed to figure out molecule(s) as well as the binding site that coordinating with PRC1. This study will provide fundamental data for clinical aneuploidy oocyte screen.