青稞膳食纤维对多酚生物利用的作用机制

青稞作为云南省迪庆藏族自治州传统种植作物，研究开发相对薄弱。基于谷物膳食纤维作为多酚化合物的重要传递物质，对多酚生物利用度有重要影响，本研究首次以不同比例青稞SDF/IDF为材料，以阿魏酸为生物标记物，通过体外模拟胃肠道消化、结肠发酵以及动物实验，利用比色法、色谱法和质谱法测定各阶段样品中多酚化合物含量、组成、回收率和抗氧化活性，并进行短链脂肪酸分析和药物动力学研究，确定多酚化合物的生物有效性和生物利用度；结合扫描电镜和傅立叶变换红外光谱，研究膳食纤维与多酚的类型、分子量和结构对其复合物结合强度的影响，并确定其作用位点、类型和分子构造，研究复合物与多酚生物有效性的构效关系，阐明膳食纤维和多酚相互作用对多酚生物利用度的作用机制，为提高多酚化合物的生物有效性和生物利用度，持续发挥抗氧化作用，维持机体健康提供科学依据，同时为青稞开发利用提供思路，促进云南省青稞产业发展。

Hulless barley is one of the traditional planting crops in Diqing Tibetan Autonomous Prefecture, Yunnan Province. Less research hinders the development of hulless barley industry. Cereal dietary ?bre is a natural functional ingredient to deliver phenolic compounds into the gut. The interactions of hulless barley dietary fibers with phenolic compounds play important role in the bioaccessibility and bioavailability of polyphenols in vivo. Using ferulic acid as the biomarker, different ratios of SDF/IDF are subjected to in vitro enzymatic digestion, microbial fermentation and animal experiment in order to determine the bioaccessibility and bioavailability of their phenolic compounds. The total phenolic contents，recovered phenolic contents，phenolic compounds, antioxidant activities and short chain-fatty acids are determined by colorimetry, chromatography and mass spectrometry. The in vivo pharmacokinetics researches carry out through the animal experiment.Furthermore, the effects of types of dietary fiber and polyphenol, ratios of SDF/IDF, molecular structure of dietary fibers and polyphenols, molecular conformation and bonding strength of dietary ?bre-phenolic compounds on the bioaccessibility of phenolic compounds are investigated by means of scanning electron microscope and fourier transform infrared spectroscopy. The object of this study is to investigate the structure-activity relationship between dietary ?bre-phenolic compounds and the bioaccessibility of polyphenols, and to elucidate the action mechanism of bioavailability of polyphenols, in order to provide scientific basis for enhancing the bioaccessibility and bioavailability of phenolic compounds, maintaining the antioxidant capacity, and keeping a healthy body. Also, it can provide ideas for the exploitation of hulless barley and promote the development of hulless barley industry of Yunnan Province.