腹泻性贝毒（DSP）是一类常见的贝毒素，可引起腹泻性中毒，并有引发肿瘤的危险。但贝类对DSP毒素有一定的耐受性，且不同贝类对其毒性的响应存在一定差异。我们推测，DSP毒素可能通过贝体内Nrf2/ARE信号通路激活体内代谢解毒酶，减轻毒素对贝的毒性。本项目拟通过贝类不同组织中Nrf2表达的分析、DSP毒素染毒对贝类不同组织Nrf2及其Nrf2/ARE信号通路下游解毒相关基因表达的影响研究、Nrf2/ARE信号通路受到影响时贝类对毒素响应的变化分析，以及不同贝类Nrf2/ARE信号通路对DSP毒素的响应比较等，阐释Nrf2及其Nrf2/ARE信号通路下游解毒基因的表达、毒素积累以及贝类对DSP毒素响应的关系，揭示贝类Nrf2/ARE信号通路参与DSP毒素代谢解毒的分子机制，为进一步阐明毒素在贝体内代谢解毒的分子机制，厘清不同贝类对毒素响应差异的原因奠定基础，为贝类海产品贝毒的脱除提供新的思路。

Diarrhetic shellfish poisoning (DSP) toxins, one of the most important shellfish toxins, can lead to diarrhetic poisoning of humanity, and moreover, induce tumor. However, bivalves have some tolerance to DSP toxins, and various bivalve species exhibit different sensitivities to the toxicity of DSP toxins. Nrf2 is the main transcription factor that mediates ARE driven transcription. It plays an important role in protection against oxidative stress and chemicals. In this study, the Nrf2 expression in different tissues of the bivalve Perna viridis will be firstly analyzed. Secondly, the expressions of Nrf2 and related Nrf2/ARE regulated detoxification genes in bivalve will be observed upon exposure to DSP toxins. Thirdly, the expressions of Nrf2/ARE regulated detoxification genes, DSP toxins contents and responses of the bivalve to the DSP toxins will be analyzed when Nrf2 is restrained or activated. Finally, the responses of Nrf2/ARE signaling pathway to the DSP toxins in the three bivalve such as P. viridis, Crassostrea ariakensis and Ruditapes philippinarum will be compared. Our project will provide new insights into the detoxification of DSP toxins in bivalve species, and give some clues for DSP toxins removal.