钙离子对质膜NADPH氧化酶（PM-NOX）活性具有调节作用，而PM-NOX参与了低浓度T-2毒素诱导马铃薯块茎活性氧（ROS）积累和对干腐病的抗性，但参与该过程的PM-NOX种类、活性以及在质膜ROS积累中的作用尚不明了。本研究拟选用马铃薯块茎为试材，综合采后病理学、生理生化、细胞生物学和分子生物学等多学科的方法，通过T-2毒素结合Ca2+处理，利用显微观察、生化测定、实时定量PCR（RT-PCR）、iTRAQ结合LC-MS/MS、Western blot蛋白质免疫印迹等技术，在分子、生化和细胞水平上解析参与T-2毒素诱导块茎质膜ROS积累的PM-NOX的基因类型及蛋白种类，明确PM-NOX参与T-2毒素诱导马铃薯块茎质膜ROS积累的分子机制，为进一步揭示质膜ROS积累在T-2毒素诱导马铃薯块茎抗病反应中信号转导机制奠定理论基础。

Calcium ion plays an important role in adjusting the activity of NOX, plasma membrane NADPH oxidase (PM-NOX) has involved in reactive oxygen species (ROS) accumulation and resistance against dry rot induced by T-2 toxin at low concentration in potato tuber. However, the types of PM-NOX, activities, and function in ROS production remain unknown. In this proposal, potato tubers are chosen as material, microscopy observation, biochemistry analysis, RT-PCR detection, isobaric tags for relative and absolute quantitation (iTRAQ)-LC-MS/MS and Western blot are employed to reveal the types of PM-NOX gene and proteins implicated in ROS accumulation in potato tubers induced by T-2 toxin and calcium ion with the help of the multidisciplinary approaches involving the postharvest pathology, cell biology, physiological and biochemistry, and molecular biology at the level of molecular, biochemistry and cellular to explain the mechanism of ROS accumulation mediated by PM-NOX and proteins of plasma membrane involved in ROS accumulation. The objects of this proposal can provide theory basis of the function of ROS signal transduction in potato tuber by T-2 toxin.