抗菌肽具有抗感染和免疫调节作用，是理想的抗生素替代物。项目组前期研发的基因工程鲎素（TPI），其作为抗菌活性分子和天然免疫效应分子可提高动物肠道菌群免疫稳态，但作用机制不明确。本项目拟以无特定病原菌（SPF）的鸡肠道为研究对象，建立SPF鸡TPI饲喂模型和SPF鸡肠炎沙门菌（SE）人工感染模型，用MiSeq高通量测序技术分析比较模型间肠道菌群演替及菌群平衡的差异，发现产生差异的效应部位和效应菌群；用免疫学和分子生物学技术系统测定和比较模型间肠道黏膜免疫平衡的差异，发现产生差异的关键免疫细胞、免疫分子和免疫调节相关信号转导通路的变化。综合分析和挖掘TPI调节肠道菌群和肠道黏膜免疫平衡共有的效应物质和效应途径，建立两种平衡的关联性，揭示TPI调节鸡肠道菌群黏膜免疫稳态的途径，预期结果不仅可以系统阐述鲎素调节鸡肠道菌群免疫稳态的分子机制，也为深入解析抗菌肽与机体相互作用关系奠定基础。

Antimicrobial peptides has anti-infection and immune regulation effect, thus it may be effective substitutes to conventional antibiotics. The early stage of the project research showed that TPI as an antibacterial and an innate immunity molecule can improve the animal intestinal homeostasis, but which mechanism is not clear. This project intends to establish Specific Pathogen Free (SPF) chicken TPI feeding model and SPF chicken enteritis salmonella (SE) artificial infection model, and to find effector molecule and flora using high-throughput sequencing technology to compare microbial selection and substitution between two models; then study homeostasis associated immune cells, molecules and signaling pathways influenced by TPI and intestinal flora; finally, to explain the TPI regulate intestinal flora to maintain gut homeostasis mechanism. This study will provide some basic mechanisms for the interaction between antimicrobial peptides and host healthy.