RNA G-四链体基因微阵列组学检测及其在恶性肿瘤细胞中组学分布特性研究

人体细胞转录产物中富含鸟嘌呤的RNA序列能够形成稳定的G-四链体结构，这些G-四链体结构的形成参与一系列重要生理功能的调控，与恶性肿瘤发生发展密切相关。本项目计划以恶性肿瘤细胞中的转录组RNA G-四链体为研究对象，设计高灵敏、高特异性RNA G-四链体识别探针，并结合基因微阵列芯片杂交技术，在组学水平实现对于恶性肿瘤转录组中系列RNA G-四链体的精确检测，系统研究数种恶性肿瘤细胞转录组RNA G-四链体的组学分布特性。并进一步通过深入探究数种恶性肿瘤细胞与正常细胞的RNA G-四链体组学分布差异性，为发展基于RNA G-四链体的新型恶性肿瘤早期检测标志物提供理论及数据基础。

Guanine(G)-rich nucleic acids sequences from human cell transcriptome can form stable G-quadruplex structures. The RNA G-quadruplex structures associate with regulation of many important biological processes and related with malignant tumor progression. This project focus on the RNA G-quadruplex omics of malignant tumor cell and plans to establish a method to determine the distribution of RNA G-quadruplex in malignant tumor transcriptome based on selective RNA G-quadruplex probes and gene microarray. This project also wants to compare the differences of RNA G-quadruplex omics between malignant tumor cells and normal tissue cells and may provide substantial basis and techniques to develop novel RNA G-quadruplex based biomarkers for early detection of malignant tumors.