基于极性快速分析法的亚硝胺前体物解析技术体系研究与应用

识别亚硝胺前体物是控制水处理中亚硝胺类消毒副产物生成的重要基础。但是 亚硝胺前体物的成分十分复杂，种类较多，研究难度很大；尤其是缺乏具有高度选择性的解 析方法一直是亚硝胺前体物研究的瓶颈。本项目在全面分析已知亚硝胺前体物的共同结构性 质的基础上，将极性快速分析法用于亚硝胺前体物的富集和分类，用不同针对性的固相萃取小柱来实现对亚硝胺前体物基本特性（具有二烷基胺官能团、极性/亲水性、带正电）的定性和定量响应；并与分子量分级技术、紫外和可见光谱、三维荧光光谱、气相色谱、液相色谱和串联质谱等分析技术联用，建立亚硝胺前体物解析技术体系，在实际水样测试中进行检验和校正。该解析技术体系有望更精确地定位复杂有机物基质中的亚硝胺活性成分，研究亚硝胺前体物各组分在水处理工艺过程中的变化，为供水行业应对亚硝胺问题提供重要借鉴。

It is very essential to understand the nitrosamine precursors for effective controlling the formation of these carcinogenic disinfection by-products in water and wastewater treatment. However, the lack of highly-selective and sensitive isolation and fractionation tool has been the bottleneck in study of nitrosamine precursors due to their complexity and diversity. This proposal suggests establishing a promising characterization tool for nitrosamine precursor isolation and fractionation based on the polarity rapid assessment method (PRAM). PRAM would make use of different selective solid phase extraction cartridges to catch the nitrosamine precursors which have been concluded as polar, hydrophilic, positive charged and containing the secondary amine functional group. Other technique, such as ultrafiltration membrane separation, UV-visible spectroscopy, 3D EEM fluorescence, gas chromatography, liquid chromatography and tandem mass spectroscopy will also be applied to fractionate and explore the structure of nitrosamine precursors. This combined fractionation technology will be tested and modified by real water samples. This combined fractionation technology could locate the dense conglomerate of nitrosamine precursors more precisely in the complex organic matrix and illustrate their dynamic along the water treatment processes, which eventually benefit the water engineers to control nitrosamine yield in the finished water.