植物在逆境下产生活性氧，诱导抗氧化酶活性升高、抗逆基因表达上调。条斑紫菜为潮间带典型物种，高盐胁迫下细胞内脱落酸（ABA）含量显著增加,ABA间接合成途径的两个关键酶及与ABA受体相互作用的激酶表达上调；外源添加ABA可诱导抗氧化酶活性升高。提示条斑紫菜中存在ABA介导的抗氧化代谢通路。本项目拟通过高盐处理，基于抗氧化酶活及ABA含量检测，获得ABA信号转导活跃的材料，开展转录组与蛋白组表达分析，通过生物信息学比对筛查ABA信号转导关键蛋白；克隆其基因并制备抗体；采用酵母双杂交系统筛选cDNA 表达文库中与诱饵蛋白相互作用的分子；免疫共沉淀验证,质谱鉴定，寻找与目标蛋白发生相互作用的分子；采用某些关键蛋白的拮抗/抑制剂，活体水平验证目标蛋白在抗氧化胁迫响应中的作用。项目的完成，可为ABA介导的逆境响应机制演化提供数据支持，为条斑紫菜抗性品系的培育提供新思路。

Active oxygen radicals will be induced when the plant is subjected to environmental stresses. The burst of ROS usually leads to the up-regulation of genes related to stress responding and the increase of antioxidases activity. Our previous investigations found that the ABA content in high salinity treated Py. yezoensis showed a significant up-regulation compared with the in the control. The enzymes relating to ABA synthesis, and the kinase interacting with the ABA receptor were up-regulated. Applying of exogenous ABA, the damage caused by malondialdehyde (MDA) was alleviated, and the antioxidases activities were enhanced. All the results implied the presence of ABA-mediated antioxidative metabolism in Py. yezoensis. According to the determinations of ABA content and antioxidant enzyme activity, the samples with active stress responding will be collected to perform analysis of transcriptome and proteome. The genes which are relevant to ABA signal transduction will be obtained through blasting. For the proteins with pivotal function in ABA signal transduction will be cloned and the monoclonal antibody will be prepared. The key protein as a bait is recombined into the GAL4 DNA-binding domain and libraries of prey proteins are constructed using RNA extracted from the stressed Py. yezoensis. The yeast two-hybrid screen will be carried out and the gene information in positive clone will be sequenced. The Co-Immunoprecipitation method will be applied to test the interaction between molecules identified by the yeast two-hybrid system. The potential role of the regulatory molecule in ABA signal transduction can be analyzed through determination of the photosynthetic parameters of Py. yezoensis with or without antagonists of the target protein under salinity stress. Thus, the molecular mechanism of antioxidation mediated by ABA in Py. yezoensis could be constructed. This will provide new information for evolutionary analysis of ABA signal transduction and the theoretical principle for the breeding of Py. yezoensis.