水稻种子耐贮性基因SS1的鉴定与功能分析

种子耐贮性是指在一定贮藏条件下种子能够保持生活力所经历的时间。它与作物种子质量和种子衰老密切相关，是一种重要的农艺性状和生物学性状，但其分子遗传基础尚不清楚。本项目拟利用水稻（Oryza sativa L.)核心种质资源、9311和Nipponbare衍生的重组自交系以及染色体片段代换系材料开展种子自然老化和人工加速老化试验，评价它们的种子耐贮性特点，全基因组定位种子耐贮性的数量性状位点，系统开展种子耐贮性的遗传基础研究；鉴定并克隆一个主效耐贮性基因SS1，综合采用生理学、分子生物学以及代谢组学等手段，剖析SS1调控种子耐贮性的分子机理；利用核心种质挖掘种子耐贮性的有利等位基因。预期结果将为明确种子耐贮性的分子调控机理奠定基础，为水稻种子耐贮性的遗传改良提供优异的种质资源，为作物种子的合理有效贮藏提供理论和实践依据。

Seed storability, defined as the seed viability after storage, represents a complex trait of agronomical and biological importance in crops. However, the genetic and molecular basis of seed storability is unclear. To investigate the genetic and molecular basis of seed storability in rice, this proposed project will use three types of rice populations in an integrated way to evaluate the viability of seeds treated under artificial aging and conventional storage conditions, and to identify quantitative trait loci (QTL) associated with seed viability. These populations include a panel of rice core germplasm, a set of recombination inbred lines and chromosomal segment substitution lines that were derived from a cross between two common varieties Nipponbare and 9311. We will verify and functionally analyze a major QTL (SS1) for seed storability, and discover the genetic network and molecular mechanisms regulating seed storability in rice, by jointly applying molecular biological, biochemical and metabolic approaches. We will also explore the natural occurring alleles and its function diversity in the rice core collection. The expected output will enhance our understanding of the genetic and molecular mechanisms underlying seed storability and provide a theoretical and practical basis for the efficient improvement of seed storability in crops.