为了确定辣椒低温响应液泡膜水通道蛋白基因CaAQP在逆境胁迫中的表达特征及功能，本项目拟在获得该基因全长cDNA序列，明确该基因在低温胁迫过程中呈下调表达趋势，原核表达能诱导该蛋白的基础上，构建该基因的超量表达载体及RNA干涉载体，同时转化辣椒品系P70，获得不同载体的辣椒转基因植株群体。在此基础上，重点研究以下内容：①分析不同逆境条件下（低温、干旱、高盐及疫霉菌侵染）辣椒水通道蛋白候选基因CaAQP的表达谱，明确其表达模式与抗逆性的关系；②比较超量及抑制表达该基因后转基因植株在诸逆境胁迫中的表现差异。通过以上研究，明确该基因在辣椒逆境胁迫中的表达特征及所起的作用，探讨该基因的作用机理，为辣椒及其他作物的抗逆育种提供新的基因资源和思路方法，具有重要的理论意义和巨大的潜在应用价值。

In order to determine the expression characteristic and function of the pepper low-temperature response gene CaAQP in stress, this study will construct over-expression and RNAi vocters, transform the pepper line P70 and obtain the transgenic plants about different vocters when obtained this gene's cDNA sequence, known the expression trend of this gene was dowm-regulated under chilling stress and the fusion protein can be induced expression in E. coli by Prokaryotic expression system. Base on these, the research will focus on: ① analyse the expression profile of CaAQP gene in different stress (chilling, drought, salt stress and infected by Phytophthora capsic), in further to understand the relationship between expression patterns and stress resistance; ② compare the differences about the over-expression and RNAi transgenic plants in different stress. Throught these research, the results would provide the scientific foundation for recognizing the mechanism of the aquaporins gene in stress tolerance. Also, they will provide a useful way and new material for stress tolerance breeding about pepper and other crops. In addition they will improve the theoretical research and application level of plant breeding.