番茄斑萎病毒（TSWV）寄主范围广，感染TSWV的植物多产生系统性坏死症状，因此造成巨大的经济损失。申请人拟在初步鉴定TSWV编码的核衣壳蛋白N为病毒导致系统性坏死症状决定因子的前期工作基础上，通过分子生物学、生物化学、细胞生物学和植物病毒学等多学科手段研究TSWV N诱导的植物系统性坏死的特性包括其与N蛋白积累量、环境因素及植物PCD死亡途径的关系，鉴定N诱导系统性坏死的关键结构域，探明N诱导的系统性坏死与其自身存在方式的关系，鉴定N诱导死亡的寄主靶标因子及诱导死亡的作用机理，进而揭示TSWV N诱导植物系统性坏死的致病分子机制。本项目的实施对于揭示TSWV侵染植物时产生系统性坏死症状的致病分子机制具有重要的科学意义，同时也将有助于病害的防控。

Tomato spotted wilt virus (TSWV) is distributed across the world with a broad host range, causing severe economic loss worldwide due to virus resulted systemic necrosis. Our preliminary studies showed that the N protein encoded by TSWV is the determinant of virus caused systemic necrosis. Based on this finding, this project intends to characterize the systemic necrosis caused by TSWV N such as the correlation between the N resulted systemic necrosis and its accumulation, environmental conditions and plant programmed cell death (PCD) pathway, to determine the key motif of N leading to systemic necrosis, to reveal whether the systemic necrosis is caused by the monomer or the multimers of N , to identify host factors interacting with N during virus infection, to explore the molecular mechanism of TSWV N induced systemic necrosis. The scientific significance of this project will help to understand the molecular mechanism of TSWV caused systemic necrosis, and help to prevent and control the disease.