Complete genome sequence of a lettuce chlorosis virus isolate from China and genome recombination/rearrangement analysis
Complete genome sequence of a lettuce chlorosis virus isolate from China and genome recombination/rearrangement analysis
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中国一株生菜失绿病毒全基因组序列及基因组重组/重排分析
DOI：
10.1007/s00705-017-3604-x
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发表时间：
2018-03
期刊：
影响因子：
--
通讯作者：
Xiaorong Tao
中科院分区：
文献类型：
--
作者：
Xiaohui Zhao;Min Zhu;Qian Wu;Jing Zhang;Yi Xu;Xiaorong Tao
我们测定了一种来自中国的莴苣褪绿卷须病毒（LCV-NJ）的全基因组序列。LCV-NJ的基因组由RNA 1和RNA 2组成，RNA 1和RNA 2分别为8165和8454个核苷酸。LCV-NJ RNA 1的基因组结构类似于LCV-California的基因组结构，LCV-California是一种在RNA 1中具有四个开放阅读框（ORF）的分离物。虽然LCV-NJ和LCV-California的ORF 1a和1b的氨基酸序列具有92%和99%的同源性，但LCV-NJ的ORF 2和ORF 3与LCV-California的ORF 2和ORF 3的同源性分别仅为63%和71%。此外，LCV-NJ RNA 2含有9个ORF，而LCV-California中含有10个ORF。由于LCV-NJ RNA 2的3 ′-末端区域内的173-nt序列的缺失，ORF 10缺失。在RNA 1和RNA 2的其他区域也观察到不同长度序列的插入或缺失。基于这些发现，我们建议LCV-NJ/LCV-California可能经历了基因组重组和/或在RNA 1和RNA 2重排。
We determined the complete genome sequence of a lettuce chlorosis crinivirus (LCV) from China (LCV-NJ). The bipartite genome of LCV-NJ consists of RNA1 and RNA2 which are 8165 and 8454 nucleotides (nt) in length, respectively. The genomic structure of LCV-NJ RNA1 resembles that of LCV-California, an isolate with four open reading frames (ORFs) in RNA1. Although the amino acid sequences of ORF 1a and 1b have 92 and 99% identity between LCV-NJ and LCV-California, ORF 2 and ORF3 of LCV-NJ share only 63 and 71% identity with those of LCV-California, respectively. In addition LCV-NJ RNA2 contains 9 ORFs, compared to 10 ORFs in LCV-California. ORF10 was missing due to the deletion of a 173-nt sequence within the 3’-terminal region of LCV-NJ RNA2. Insertion or deletion of sequences of varying lengths was also observed in RNA1 and other regions of RNA2. Based on these findings, we propose that LCV-NJ/LCV-California may have undergone genome recombination and/or rearrangement in RNA1 and RNA2.
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