养蚕业一直是我国的重要产业，而蚕病的危害每年都给蚕丝业带来巨大的经济损失，其中尤以家蚕血液型脓病为主。家蚕核型多角体病毒（BmNPV）是血液型脓病的病原，因其侵染家蚕的机制未被完全解析，严重阻碍了家蚕抗NPV品种的培育。本项目围绕“宿主（家蚕）与杆状病毒（BmNPV）相互作用的分子机制”这一重要科学问题，以昆虫重要的先天免疫应答——细胞凋亡为切入点开展。项目拟首先通过生物信息学和分子生物学等手段鉴定凋亡抑制基因（IAPs），并分析其结构和表达特征；然后通过在家蚕细胞中过表达和敲除目的基因，鉴定其对宿主细胞凋亡过程的影响；最后通过免疫共沉淀等技术鉴定蛋白质之间的相互作用，阐释IAPs基因在BmNPV侵染家蚕过程中的分子调控机制，并在个体水平上验证其功能。通过本项目的研究，可以进一步阐释BmNPV的致病机理和家蚕机体免疫保护机制，并为家蚕抗BmNPV病毒品种的培育提供理论支撑和素材。

The sericulture has been an important industry in our country. However, the silkworm diseases cause huge economic losses of silkworm industry each year, especially the Bombyx mori nucleopolyhedrosis. Bombyx mori nucleopolyhedrosis virus is the pathogen of nucleopolyhedrosis. Due to the molecular mechanisms of BmNPV infection has not been completely resolved, the antiviral breeding process of silkworm was hampered. This project will embroider on the important scientific problem “The molecular mechanism of the interaction of the host (Bombyx mori) and baculovirus (BmNPV)”, and we use the apoptosis, an important insect innate immune response, as the breakthrough point for studying. First, we will identify the inhibitor of apoptosis genes and analyze their structural and expression characteristics using bioinformatics and molecular biology methods. Then, we identify their impacts on the process of host cell apoptosis through the over-expression and knock-out of the target genes in the Bombyx mori cell lines. Finally, we will identify the interaction between proteins by the co-immunoprecipitation and elucidate the molecular regulatory mechanism of IAP genes in the process of BmNPV infecting of the silkworm, and verify their functions at the individual level. Through the studies in the project, the pathogenic mechanism of BmNPV and the silkworm immune protection mechanism will be further explained, and some important theoretical supports and materials will be provided for breeding the anti-BmNPV silkworm strains.