Aux/IAA在山核桃嫁接成活过程中的作用机理研究

Aux/IAA 基因作为生长素早期诱导表达的基因，在生长素信号传导和植物的生长发育中起到十分重要的作用。申请人利用克隆到一个山核桃Aux/IAA 同源基因，并初步证明了该基因可能对山核桃嫁接成活起到基因表达调控的作用。本项目拟通过克隆Aux/IAA 基因全长和启动子序列，利用定量RT-PCR、Western 杂交和免疫胶体金定位技术等方法对山核桃Aux/IAA 进行表达特性和功能研究，来阐明这一基因对山核桃嫁接成活的调控机理及其与维管束桥形成的相互关系，不仅丰富了Aux/IAA 基因家族的基因信息，为Aux/IAA 的研究提供了良好的物质基础，而且有助于揭示Aux/IAA 影响山核桃嫁接成活的作用机制和山核桃嫁接过程生长素信号传导机制，为山核桃等木本植物园艺化栽培提供一定的理论基础。

Aux/IAA gene plays an essential role in phytohormone auxin signal transduction and plant growth and development as one of the early auxin response genes. An Aux/IAA in hickory (Carya cathayensis) during graft process was cloned using cDNA-AFLP. We found that Aux/IAA play a role of gene expression and regulation during graft process. In this project, we will use the molecular biology and immuno-gold technique to observe the effect of Aux/IAA on grafting, illustrate the role of this gene on the combined effect of rootstock-scion and clarify its relationship with the formation of vascular bridge from three levels of whole - tissue - cells. It will not only enlarge the information of the Aux/IAA gene family and provide a basis for Aux/IAA research, but also benefit to uncover the mechanism of auxin across the membrane and the function of Aux/IAA in hickory during graft process, and provide a theoretical basis for horticulture cultivation of hickory and other woody plants.