果实表皮蜡质对病原真菌侵染结构形成具有诱导作用。我们研究发现梨果皮蜡质疏水性与Alternaria alternata附着胞形成率呈显著正相关，且PLC参与其诱导过程，但具体调控机制不清。本项目在对梨果黑斑病菌A.alternataPLC基因克隆及其在蜡质疏水性诱导附着胞形成过程中特异性表达分析的基础上，通过PLC抑制剂或外源钙处理，采用显微技术和生物化学、分子生物学方法研究PLC对不同疏水性表面诱导A.alternata附着胞形成过程中形态结构变化的影响；分析比较A.alternata附着胞形成过程中胞内Ca2+的动态变化及钙信号途径关键调控蛋白基因表达差异，以揭示PLC介导的钙信号途径对梨果皮蜡质疏水性诱导A.alternata附着胞形成的调控机制，为进一步从分子水平上阐明钙离子信号通路对A.alternata附着胞形成的调控机制和开发梨果采后病害新的控制方法提供理论依据。

Fruit cuticular wax have important role in inducing infection structure formation of fungal pathogen. Our previous results indicated that there was significant positive correlation between pear cuticular wax hydrophobicity and appressorium formation rate of Alternaria alternata, and PLC involved the inductive process, however the detailed mechanism remains unclear. In this study, based on PLC gene cloning and its specific expression during appressorium formation of A. alternata, causal agents of pear black spot, induced by different hydrophobicity, the effect of PLC on morphological structure changes during appressorium forming process induce by pear wax hydrophibicity were studied by the means of microscopic observation. In addition, changes in cytosolic Ca2+ and differential genes expression of key regulatory proteins in calcium/ calmodulin-dependent signaling pathway during appressorium forming process were also analyzed by the biochemical and molecular techniques. Based on the above experiments, the regulatory mechanisms of PLC mediated calcium/calmodulin-dependent signaling pathway on appressorium formation induced by pear wax hydrophobicity will be systematically clarified. The results will provide a theoretical basis for further understanding mechanism of calcium/ calmodulin-dependent signaling pathway on appressorium formation of A. alternata and exploiting novel strategies for intervention of postharvest diseases in fruit and vegetables.