microRNA是一类长为22-23 nt、具有重要转录后调控作用的非编码RNA。蚊子因为吸血成为传播许多疾病的媒介昆虫。研究蚊子吸血和营养信号传导的分子机制对于寻找防治蚊子的新方法至关重要。我们已发现吸血蚊子所特有和中肠特异性的miR-1174通过调节其靶基因丝氨酸羟甲基转移酶(SHMT)来控制蚊子吸血、血液消化、卵巢发育和蚊子的寿命。在此基础上，将鉴定miR-1174的调控序列，深入研究其转录表达机制，利用基因组编辑技术、转录组和蛋白组组学方法与分子杂交等常规技术深入研究miR-1174与其靶基因之间的精细调控关系以及它们对蚊子吸血、血液消化吸收和营养信号传导的分子机制。利用基因组编辑技术获得转基因工程蚊子，为野外防控蚊子建立实验模型。总之，对miR-1174和其靶基因在调控蚊子吸血和营养信号传导方面的深入研究具有重要的理论价值和现实意义，可能会影响今后控制病原媒介昆虫的策略。

MicroRNAs (miRNAs) are noncoding 22- 23 nt RNAs that usually post-transcriptionally regulate protein-coding genes through perfectly or imperfectly pairing with complementary sites within the 3′-untranslated region (UTR) of mRNAs. Female mosquitoes require a blood meal for reproduction, thus serve as vectors of many devastating human diseases. A deeper understanding of the molecular mechanisms linked to mosquito blood meal processes and transduction of trophic signals is of particular importance for devising innovative vector control strategies. We have discovered that the mosquito- and midgut-specific microRNA 1174 controls normal sugar absorption, fluid excretion, blood intake, and, consequently, egg maturation and survival in female mosquitoes by targeting serine hydroxymethyltransferase (SHMT). These results lay the important clues for further exploring the molecular mechanisms of mosquito gut physiology. Based on our previous work, we will identify the promoter of this mosquito-specific miRNA, and reveal the mechanism in its own transcriptional expression. Most importantly we will focus on the fine regulation relationship between miR-1174 and its target SHMT or other potential target genes involved, and the fine molecular mechanisms by miR-1174 and SHMT involved in blood taking, blood digestion and absorption, and transduction of trophic signals, by using the genome editing technique CRISPR/CAS 9, comparative transcriptomics and proteomics, and other conventional bio-molecular techniques like molecular hybridization. By means of genome editing technique, we will also set up the transgenic model mosquitoes for the vector control. Summarily, in-depth knowledge of the molecular mechanisms involved by mosquito-specific miRNA 1174 and its target(s) in blood sucking and transduction of trophic signals has great theoretic and practical implications, and will hopefully influence the future tactics in vector control.