神经病理性疼痛是常见的难治性疾病，发病机制复杂。国外研究发现：高频脊髓电刺激对神经病理性疼痛疗效显著，但机制不明。课题组前期对大鼠在体坐骨神经阻滞及压迫性损伤（CCI）模型研究中发现，低阈值的千赫兹高频电刺激可减轻模型大鼠对机械痛敏感性，且脊髓背根神经节（DRG）中Nav1.3表达降低。结合高频电刺激物理特性，我们推测其机制可能是：高频电场引起了离子快速震荡位移、通道蛋白构象变化及神经细胞膜动力学异常，由此抑制了受损神经异常放电，并阻断了神经轴索运输，从而缓解神经病理性疼痛。我们拟在预实验基础上，应用千赫兹高频电刺激CCI大鼠坐骨神经，通过探究大鼠DRG中Nav1.3和Nav1.8离子流、脊髓背角的NMDA受体通道离子流、脊髓背角浅层场电位变化、胶质细胞激活情况及DRG中CDNFmRNA和NGFmRNA表达的变化，验证千赫兹高频电刺激治疗神经病理性疼痛机制假说，为临床应用提供理论依据。

Neuropathic pain is the most common refractory disease with complicated pathogenesis. At present, the lack of effective treatment has become an important clinical problem to be solved urgently. Exploratory studies abroad have found that kilohertz high-frequency spinal cord electrical stimulation is effective in the treatment of neuropathic pain, but the mechanism remains unknown. Our research group established a subthreshold intensity high-frequency electrical stimulation model for treating rat sciatic nerve compression injury (CCI) on the basis of previous studies on high-frequency electrical stimulation of rat sciatic nerve conduction block in vivo. It has been found that kilohertz high-frequency electrical stimulation can reduce the sensitivity of model rats to mechanical pain, and the expression of Nav1.3 in DRG is reduced. Based on the high-frequency electrophysiological characteristics, we speculate that the treatment of neuropathic pain may be attributed to ion oscillation displacement, channel protein conformation change and nerve cell membrane dynamics abnormality caused by high-frequency electric field, inhibiting abnormal discharge of damaged nerve and blocking axonal transport to relieve neuropathic pain. On the basis of pre-experiments, we intend to verify the therapeutic effect of kilohertz high-frequency electrical stimulation through the changes of Nav1.3 and Nav1.8 ion flow in rat DRG, NMDA receptor channel ion flow in spinal dorsal horn,superficial field potential of spinal dorsal horn，glial cell activation and CDNFmRNA and NGFmRNA expression in DRG before and after kilohertz high-frequency electrical stimulation, which can verify the hypothesis about the mechanism of high-frequency electrical stimulation in the treatment of neuropathic pain, and provide a theoretical basis for clinical application.