环境中广泛存在的人畜共患病原菌严重威胁农产品安全、人类健康以及农业经济发展。大肠杆菌O157:H7作为常见病原菌，具有感染剂量极低、致病性高以及传播范围广等特点。目前环境中O157:H7的研究多集中在土壤/沉积物和单纯的水环境。挺水植物作为水生生态系统的重要组成，其根系微环境中O157:H7的存活尚不清楚。本项目拟通过水培和根箱试验，阐明挺水植物根系微环境特征，量化环境温度、pH和根系铁胶膜对O157:H7根部定殖的驱动作用，深入剖析O157:H7在挺水植物根系微环境中的存活特征，并进一步通过微生物人工合成群体和FlowPot系统，验证挺水植物根系微生物组的特征菌群组成，深入探讨外源O157:H7与根系微生物组间的互作机制。本项目研究有助于提升病原菌在根系微环境的研究水平，丰富和深化对外源病原菌与根系微生物组相互作用的认知，为发展病原菌污染防治和修复技术体系提供理论基础。

The widespread zoonotic pathogen in the environment can become a reservoir of future infections, which is a great threat to food safety, human health and agricultural economy development. Escherichia coli (E. coli) O157:H7, an important pathogen, is dangerous because of its low infective dose and high pathogenicity. Most of the previous studies focused on the soil/sediment and water environment. However, O157:H7 survival in root micro-environment of emerged plant, which is an important component of aquatic ecosystems, remains unclear. In this project, both the hydroponic culture and rhizobox experiment will be conducted to investigate the characteristics of emerged plant root micro-environment and its O157:H7 survival. Meanwhile, the root colonization by O157:H7 and the influence of environment temperature, pH and root iron plaque will be revealed. In addition, the synthetic community and FlowPlot system will be carried out to verify the composition of root microbiota and reveal the interaction between invading O157:H7 and root microbiota. The results of this project are expected to provide new knowledge on the environmental behavior of O157:H7 in the root micro-environment of emerged plant, and then contribute to a deeper comprehension of the interaction between the exogenous pathogen and the root microbiota, providing scientific basis for pathogen contamination prevention.