Assessing the Risks of Potential Bacterial Pathogens Attaching to Different Microplastics during the Summer-Autumn Period in a Mariculture Cage.
Assessing the Risks of Potential Bacterial Pathogens Attaching to Different Microplastics during the Summer-Autumn Period in a Mariculture Cage.
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DOI：
10.3390/microorganisms9091909
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发表时间：
2021-09-09
期刊：
影响因子：
4.5
通讯作者：
Zhang D
中科院分区：
文献类型：
--
作者：
Hou D;Hong M;Wang Y;Dong P;Cheng H;Yan H;Yao Z;Li D;Wang K;Zhang D
随着微塑料污染的持续增加，一个新出现的威胁是微塑料可能成为病原体的新型基质和/或载体。鉴于越来越多的证据表明水产养殖物种摄入了微塑料，这对水产品安全尤其令人担忧。然而，对海产养殖中与微塑料相关的病原体的潜在风险仍然知之甚少。在这里，原位孵化实验，涉及三个典型的微塑料，包括聚对苯二甲酸乙二醇酯（PET），聚乙烯（PE），聚丙烯（PP）在夏秋期间进行的海水养殖笼。基于16 S rRNA基因扩增子测序和针对水生环境中涉及的病原菌的定制数据库，进行了潜在病原体的鉴定，以评估不同微塑料附着潜在病原体的风险。与周围海水中的自由生活和颗粒附着社区相比，在微塑料相关社区中没有观察到病原体的富集。尽管相对丰度较低，但病原体对三种微塑料基质表现出不同的偏好，其中PET是病原体最青睐的，特别是弧菌、韧杆菌和埃希氏菌的潜在致病成员。此外，这些病原体在微塑料上的定殖受到环境因素的强烈影响（例如，温度，亚硝酸盐）。我们的研究结果为微塑料在海水养殖业中的生态风险提供了见解。
As microplastic pollution continues to increase, an emerging threat is the potential for microplastics to act as novel substrates and/or carriers for pathogens. This is of particular concern for aquatic product safety given the growing evidence of microplastic ingestion by aquaculture species. However, the potential risks of pathogens associated with microplastics in mariculture remain poorly understood. Here, an in situ incubation experiment involving three typical microplastics including polyethylene terephthalate (PET), polyethylene (PE), and polypropylene (PP) was conducted during the summer–autumn period in a mariculture cage. The identification of potential pathogens based on the 16S rRNA gene amplicon sequencing and a custom-made database for pathogenic bacteria involved in aquatic environments, was performed to assess the risks of different microplastics attaching potential pathogens. The enrichment of pathogens was not observed in microplastic-associated communities when compared with free-living and particle-attached communities in surrounding seawater. Despite the lower relative abundance, pathogens showed different preferences for three microplastic substrates, of which PET was the most favored by pathogens, especially potentially pathogenic members of Vibrio, Tenacibaculum, and Escherichia. Moreover, the colonization of these pathogens on microplastics was strongly affected by environmental factors (e.g., temperature, nitrite). Our results provide insights into the ecological risks of microplastics in mariculture industry.
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