基于石墨烯纳米材料的多通道微流控阻抗传感方法同步检测果蔬中多种致病菌的研究

食源性致病菌污染已成为食品安全的重大隐患之一，然而由于现有检测技术存在的各种局限性，迫切需要研究一种高灵敏度、高准确性、低成本的快速检测方法来满足目前国内致病菌检测的需求。为此，本项目拟研究基于石墨烯叉指阵列微电极的多通道微流控阻抗分析技术用于微量样品中多种食源性致病菌的快速同步检测。研究内容包括：（1）研究磁性纳米颗粒在复杂样品中对多种致病菌目标DNA的富集与分离并建立最优模型；（2）研究石墨烯叉指阵列微电极的制备工艺参数及用于阻抗分析方法检测致病菌的模型；（3）研究基于多通道微流控技术的阻抗分析方法用于微量样品中多种致病菌的同步检测，并在此基础上，建立用于实际样品分析的模型。本项目研究是对食源性致病菌检测领域的新探索，旨在形成一种新的适用于果蔬样品多种致病菌同时快速检测的方法。研究成果将有助于推动微电子技术、纳米技术和生物传感技术在食品安全领域的应用。

Recently, the contamination from foodborne pathogens has become one of the major hidden dangers in food safety. However, due to the limitations of current detection methods, the development of a sensitive, reliable, and cost-effective method for the detection of foodborne pathogens is highly desirable to the current safety requirements in our country. In this context, we aim to develope a novel impedimetric method for rapid and simultaneous detection of foodborne pathogens in trace food samples by combining graphene interdigitated array microelectrodes with multi-channel microfluidic devices. The main research contents includes: (1) To investigate the enrichment and separation of target DNAs of multi-pathogens by magnetic nanoparticles in sample matrix and establish the optimal model; (2) To investigate the process parameters in the fabrication of graphene interdigitated array microelectrodes and the impedimetric detection model based on this electrode; (3) To investigate the feasibility of impedimetric method based on multi-channel microfluidic devices for the simultaneous detection of multi- pathogens in trace sample, and establish the optical model for real samples analysis. The ultimate goal of this project is to foundation of a novel technique for the rapid and simultaneous detection of foodborne pathogens in fruit and vegetable samples. This research would accelerate the pace of application of molecular micro-electronic technique, nanotechnology and biosensing technology in the field of food safety.