可溶性晚期糖基化终产物受体（sRAGE）可抑制晚期糖基化终产物受体（RAGE）介导的细胞信号转导通路，作为保护因子阻止、延缓某些疾病的发生。血管内皮生长因子(VEGF)与PCOS发生密切相关，通过影响血管生成参与PCOS的发生。我们的预实验结果提示:sRAGE能够降低PCOS患者颗粒细胞VEGF表达,但其机制尚待进一步探讨。因此，我们提出假说：sRAGE可能通过阻断EGF相关生长因子的作用，减少PGE2产生或者通过阻断ROS-EGFR-ERK1/2通路，抑制特异性转录因子活化，下调VEGF的表达。为验证这一假说，本课题将通过人PCOS原代颗粒细胞、大鼠PCOS模型，采用RT-qPCR、Western blot、RNA干扰等手段，在分子、细胞、组织以及动物整体水平探讨sRAGE下调VEGF表达的机制，从sRAGE这个新视角为揭示PCOS发生发展机制奠定基础，为预防和治疗PCOS提供新的思路。

Soluble receptor for advanced glycation end-products (sRAGE) can inhibits advanced glycation end-products receptor (RAGE) mediated signal transduction pathways，it’s a protective factor to prevent, delay the onset of certain diseases.Vascular endothelial growth factor (VEGF) is closely related to the occurrence of PCOS by influencing the angiogenesis.Our preliminary experiment suggests that sRAGE can reduce the expression of VEGF in granulosa cells of PCOS patients ,but the mechanism remains to be further explored.Therefore, we propose the hypothesis: sRAGE possibly acts by blocking EGF-like growth factors, reducing PGE2 production, or by blocking ROS-EGFR-ERK1 / 2 pathway, inhibiting the activation of specific transcription factors, down-regulating the expression of VEGF, preventing or delaying the onset of PCOS.To verify this hypothesis, our study will use primary granulosa cells of PCOS patients, PCOS rat model, and techniques such as of RT-qPCR, Western blot, and RNA interference. To investigate the molecular mechanisms of how sRAGE down-regulate the expression of VEGF, we experiment with the molecular, cellular, tissue and animal field. From the new perspective of sRAGE, we may lay the foundation for the pathogenesis of PCOS, and further provide new ideas for the prevention and treatment of this syndrome.