杠板归是贵州特色苗药材，抗化学性肝损伤是其新功效用途，但其发挥功效的过程行为和分子作用机制不清楚，严重制约其深度开发利用。本项目以杠板归为研究对象，在传统中医药理论指导下，结合现代科学技术，对其进行系统的抗化学性肝损伤药效过程评价研究，充分弄清其发挥功效的具体过程行为与效应关系。在此基础上，引入系统生物学的先进理念—脂质组学，结合质谱成像技术，从脂质代谢角度，弄清杠板归对脂质分子代谢过程的影响，进而阐释其抗化学性肝损伤的分子作用机制。研究内容主要涉及：杠板归抗化学性肝损伤药效学过程评价、基于质谱成像技术的杠板归活性成分组织分布研究、基于 MALDI-IMS 脂质组学的作用机制研究。通过该研究，基本阐明杠板归抗化学性肝损伤的药效过程行为和分子作用机制，为其深度开发利用提供依据。此外，探索一种“脂质组学+质谱成像”的新型中药作用机制研究方法与模式，为中药民族药的合理利用及深度开发提供科技支撑。

Polygonum perfoliatum L. is a kind of Miao ethnic herb and anti-chemical liver injury is its new effect. But the process behavior and molecular mechanism of P. perfoliatum for anti-chemical liver injury are still not clear. In this project, P. perfoliatum is taken as the subject and the process behavior on anti-chemical liver injury will be systematic study under the guidance of traditional Chinese medical theory, combining with the modern science and technology. The mechanism of anti-chemical liver injury will be study from the perspective of lipid metabolism by using the concept of lipidomics and MALDI-IMS method. The research mainly involves: evaluation of the pharmacodynamic process of P. perfoliatum on chemical liver injury, distribution of active components of P. perfoliatum based on the technique of mass spectrometry imaging, and the mechanism study of P. perfoliatum on chemical liver injury based on MALDI-IMS lipidomics. Through this study, the pharmacodynamic process behavior and molecular mechanism of P. perfoliatum on chemical liver injury will be clarified, which provides the basis for its deep development and utilization. In addition, a new type of traditional Chinese medicine mechanism research method and model based on lipid metabolism combined with MALDI-IMS will be explored, which may provide a scientific basis for the rational utilization and deep development of traditional Chinese medicine.