前期本课题组利用蛋白质组学等技术筛查了大量临床肿瘤样本。其中C10orf99蛋白被我们发现在部分结直肠癌肿瘤组织间液中高表达，而在相应的癌旁组织间液中相对低表达。生物信息学分析表明C10orf99具有N端信号肽，编码仅有57个氨基酸的成熟小分子量蛋白，序列与现有其他蛋白没有相似性，其生物学功能尚无明确报道。我们进一步的实验研究表明该基因可分泌表达，在结直肠癌，肾癌，皮肤癌，头颈部肿瘤等上皮来源肿瘤组织高表达。在对结直肠癌细胞的体外研究中，我们发现且其具有明显的促进肿瘤细胞增生与侵袭的作用。本项目拟在前期基础之上，通过临床肿瘤样本分析C10orf99的表达与多种肿瘤发生、转移及预后的相关性，并进一步利用多种技术方法和动物模型，在体内外深入研究该基因在结直肠癌细胞增生，侵袭与转移中的作用及其分子机制；以期在为C10orf99的功能研究填补空白的同时，为其进一步向临床转化提供理论基础。

Previously our group performed large-scale proteomic screening of clinical tumor samples. Among them, C10orf99 was found in higher concentration in the interstitial fluid of colorectal cancer tissues, but was relatively lower in tumor-adjacent normal tissue. Bioinformatic analysis suggest that C10orf99 contain N-terminal signal peptide sequence and a mature protein of 57 amino acid. The sequence of C10orf99 is not similar with any other protein, and its biology function has not been reported till now. Our further studies indicated that C10orf99 was a secretory protein, which was over-expressed in colorectal cancer, renal carcinoma, skin cancer, as well as head and neck cancer. Moreover, we also found that it significantly promoted proliferation and invasion in colorectal cancer cells in vitro. In the present study, we plan to carried out an investigation on the correlation between the expression level of C10orf99 and tumor genesis, metastasis and prognosis. And we will have a deep investigation on the biology fuction and molecular mechanisms of this gene in the proliferation and metastasis of colorectal cancer cells, with an aim to fill the blank of the function of C10orf99 and provide the theory basis for its further clinical transformation.