试管苗玻璃化是植物组织培养中普遍发生的现象，对植物脱毒、快繁及基因遗传转化影响严重，但其发生机制至今尚未明确。研究表明，试管苗玻璃化过程中存在活性氧物质(ROS)的产生和积累、活性氧清除系统紊乱和细胞膜损伤，我们前期试验也证实ROS与试管苗玻璃化和细胞膜损伤相关，但相互关系的机理国内外迄今尚少有报道。根据相关研究和我们前期工作，我们推测，具有信号传导和氧化伤害功能的ROS在试管苗玻璃化中有重要作用，其不同功能的表达可能是调控试管苗玻璃化的新机制。为证实这一假设，我们在不同培养条件下培养试管苗,建立内源ROS产生和积累与试管苗玻璃化发生模型，采用组织观察，生理测定，转录组测序和分析等技术，从组织-细胞-分子等不同层次，探讨内源ROS对试管苗玻璃化、细胞膜结构和功能异变、相关基因差异表达的影响。本课题将从新的角度提出试管苗玻璃化的机制，为试管苗玻璃化调控奠定基础。

Hyperhydricity in plantlet in vitro, a common problem, has seriously influenced virus-free and rapid propagation in plant tissue culture,as well as gene genetic transformation. However, its mechanism remains unclear. It is shown that accumulation of reactive oxygen species（ROS）, disturbance in ROS scavenging system and damage to cell membrane were found during the process of hyperhydricity. Our previous research demonstrated that ROS was related to the hyperhydricity and oxidative damage of cell membrane. To date，the relationship between them was rarely studied at home and abroad. According to the previous reports and our studies, it would be predicted that ROS, as a signal transduction or oxidative damage substances, plays a key role in hyperhydricity, which could be the probable novel mechanism of hyperhydricity in plantlet in vitro. To prove this hypothesis, the model between the accumulation of ROS in different treatments and hyperhydricity in plantlet in vitro would be established by means of the observation of the tissue structure, the determination of physiological function and transcriptome sequencing. From the tissue, cell and molecule levels, we can analyze and discuss the effect of different ROS concentration on the hyperhydricity, the structural variation of cell membrane and function and the expression of related genes. This research will provide a new perspective of hyperhydricity mechanism and lay a foundation on controlling hyperhydricity in plantlet in vitro.