我们已证明ALV-J感染抑制细胞自噬，且ALV-J感染促进circ-Vav3海绵吸附gga-miR-375诱导肿瘤形成。研究表明肿瘤形成与自噬抑制密切相关。前期研究发现，circ-Vav3海绵吸附gga-miR-375抑制细胞自噬。那么circ-Vav3海绵吸附gga-miR-375是否介导ALV-J抑制细胞自噬？进一步实验显示：ALV-J感染下调gga-miR-375而上调CIP2A的转录，ALV-J感染抑制细胞自噬促进CIP2A表达和AKT磷酸化，且CIP2A促进AKT磷酸化修饰。据此我们提出假设：Circ-Vav3海绵吸附gga-miR-375调控CIP2A/AKT介导ALV-J抑制细胞自噬。本项目拟采用激光共聚焦等方法阐明circ-Vav3海绵吸附gga-miR-375调控CIP2A/AKT介导ALV-J抑制细胞自噬的分子机制，研究成果为防控J亚群禽白血病提供新视野。

We have demonstrated that ALV-J infection inhibits autophagy and that ALV-J infection promotes circ-Vav3 sponges gga-miR-375 to induce tumor formation. Studies have shown that tumor formation is closely related to autophagy inhibition. Previous studies have found that circ-Vav3 sponges gga-miR-375 to inhibit autophagy. So does circ-Vav3 sponge gga-miR-375 to mediate the autophagy inhibition by ALV-J infection? Further experiments showed that ALV-J infection down-regulates gga-miR-375 and up-regulates the transcriptional level of CIP2A, ALV-J inhibited autophagy to promote the protein expression of CIP2A and AKT phosphorylation, and CIP2A promoted AKT phosphorylation. Based on this, we hypothesized that ALV-J inhibits autophagy mediated by circ-Vav3 sponges gga-miR-375 regulating CIP2A/AKT. This project intends to use laser confocal and other methods to elucidate the molecular mechanism of ALV-J inhibits autophagy mediated by circ-Vav3 sponges gga-miR-375 regulating CIP2A/AKT, research results provide a new perspective for the prevention and control of subgroup J avian leukosis.