脊髓损伤继发的肠道菌群失调影响患者功能恢复及生活质量；肠道菌群及代谢产物对5-HT系统有调控作用。结合前期研究，我们提出工作假说：针刺通过调节肠道菌群及代谢产物，多靶点、多通路良性调控结肠5-HT系统，促进肠道功能恢复，可能是其治疗脊髓损伤大鼠排便障碍的重要机制之一。拟电针干预脊髓损伤排便障碍大鼠，并以对模型鼠进行电针菌群移植，在对电针和电针菌群的治疗效应进行评价的基础上；采用16SrDNA和宏基因组学高通量测序、气相色谱质谱非靶标代谢组学技术结合生物信息学分析，明确电针对脊髓损伤排便障碍大鼠肠道微生态的调节作用，并探索电针潜在治疗靶点及其代谢通路。采用ELISA、Real time-PCR、Western Blot和免疫组化技术检测肠道菌群调节的中间分子及5-HT系统各分子在结肠中的表达。探讨电针调控5-HT系统的微生态调节机制。从新视角为针灸临床康复的推广应用提供理论和实验依据。

Intestinal dysbiosis secondary to spinal cord injury affects the functional recovery and the quality of life. Intestinal microflora and metabolites regulate 5-HT system. Combined with previous studies, we hypothesize: acupuncture can regulate intestinal microflora and metabolites, and then regulate the colon 5-HT system by multi-target and multi-channel, and promote intestinal function recovery, which may be an important mechanism to treat defecation disorders in rats with spinal cord injury. This study intends to electroacupuncture (EA) on the defecation disorders rat following spinal cord injury and transplant intestinal microbiota of EA group to model rats. The treatment effect of EA and EA intestinal microbiota was assessed. To measure the influence of EA on intestinal microecology and explore potential therapeutic targets and metabolic pathways of EA, 16SrDNA，Metagenomics high-throughput sequencing，and GC-TOF-MS for metabolomics will be used. Intermediate molecules regulated by intestinal microbiota and various molecules of 5-HT system in colon were determined by ELISA, Real-time PCR, western blot, and immunohistochemistry. The purpose of our project is to reveal the microecological regulation mechanism of 5-HT system controlled by EA, and provide new theoretical and experimental basis for the popularization and application of acupuncture and moxibustion in clinical rehabilitation.